Bacillus methylotrophicus NKG-1 Wettable Powder: Preparation and Efficacy Study

doi:10.11924/j.issn.1000-6850.casb2021-0729

Abstract

Abstract:

In order to prepare wettable powder of Bacillus methylotrophicus NKG-1 and evaluate its effect on disease prevention and growth promotion, we used NKG-1 as the test material, determined the optimal formula of the wettable powder (WP) through the selection of carrier and auxiliary agent by the mixture design, and conducted the efficacy evaluation on the WP. The results showed that the best carrier of NKG-1 WP was silica, and the best additives were the mixture of alkyl naphthyl sulfonate formaldehyde condensation and block copolymer (Morwet D-500) and alkyl naphthalene sulfonate (Petro AA). The mixture design determined the optimal mass ratio: silica: Morwet D-500: Petro AA=72:16:12. The control efficacy test results showed that the fresh weight, dry weight, plant height and root length of tomato plants treated with NKG-1 wettable powder 100 times solution increased by 53.34%, 61.01%, 88.02% and 78.53%, respectively, compared with those of the control. The average control effect of NKG-1 wettable powder on Botrytis cinerea could reach 82.02%. In this study, the optimal ratio of Bacillus methylotrophicus NKG-1 WP was determined, and the efficacy of the preparation was evaluated, which could lay an experimental foundation for the production and application of NKG-1.

Key words: Bacillus methyltrophicus, preparation technology, mixture design, wettable powder, control efficacy

CLC Number:

S482.292

ZHANG Wei, LV Zhaoyang, LI Boya, WANG Cong, GE Beibei, ZHANG Kecheng, PARK Kyung Seok, SHI Liming. Bacillus methylotrophicus NKG-1 Wettable Powder: Preparation and Efficacy Study[J]. Chinese Agricultural Science Bulletin, 2022, 38(15): 130-138.

Figures/Tables 12

References 36

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载体	悬浮率/%	润湿时间/s	有效活菌数/ (10⁹ CFU/g)
高岭土	13.06±6.75b	6.36±0.84b	3.30±0.55c
硅藻土	15.86±6.83b	13.83±1.10b	4.10±0.08b
白炭黑	35.30±7.73a	96.58±12.99a	24.00±0.39a

载体	悬浮率/%	润湿时间/s	有效活菌数/ (10⁹ CFU/g)
高岭土	13.06±6.75b	6.36±0.84b	3.30±0.55c
硅藻土	15.86±6.83b	13.83±1.10b	4.10±0.08b
白炭黑	35.30±7.73a	96.58±12.99a	24.00±0.39a

助剂	有效活菌数/(10¹⁰ CFU/g)	润湿时间/s	悬浮率/%
木质素	1.97±0.08abc	128.54±3.32c	60.36±0.84a
PVA	1.87±0.10bc	124.99±5.92c	57.24±0.34b
Morwet D-425	1.93±0.07abc	69.34±5.62a	56.40±0.19c
Morwet D-450	2.07±0.11abc	91.73±17.02b	53.26±0.20d
Morwet D-500	2.14±0.08ab	69.85±0.26a	56.52±0.04c
Agrilan 700	1.74±0.11c	153.20±23.92d	57.70±0.05b
Berol 790A	1.99±0.47abc	75.87±7.84ab	53.53±0.28d
Petro AA	2.23±0.08a	63.20±2.76a	56.35±0.02c
Morwet EFW	1.89±0.06abc	65.44±4.07a	57.45±0.03b
对照	2.40±0.39a	96.58±12.99b	35.30±7.73e

助剂	有效活菌数/(10¹⁰ CFU/g)	润湿时间/s	悬浮率/%
木质素	1.97±0.08abc	128.54±3.32c	60.36±0.84a
PVA	1.87±0.10bc	124.99±5.92c	57.24±0.34b
Morwet D-425	1.93±0.07abc	69.34±5.62a	56.40±0.19c
Morwet D-450	2.07±0.11abc	91.73±17.02b	53.26±0.20d
Morwet D-500	2.14±0.08ab	69.85±0.26a	56.52±0.04c
Agrilan 700	1.74±0.11c	153.20±23.92d	57.70±0.05b
Berol 790A	1.99±0.47abc	75.87±7.84ab	53.53±0.28d
Petro AA	2.23±0.08a	63.20±2.76a	56.35±0.02c
Morwet EFW	1.89±0.06abc	65.44±4.07a	57.45±0.03b
对照	2.40±0.39a	96.58±12.99b	35.30±7.73e

助剂	添加比例/%	润湿时间/s	悬浮率/%
Morwet D-425	4	58.11±1.82d	57.85±0.37h
	8	87.57±6.41f	70.41±1.17c
	12	46.01±1.75b	65.44±0.75e
Morwet D-500	4	40.03±0.61ab	55.17±0.01i
	8	39.62±1.67a	68.13±0.92d
	12	37.01±2.05a	77.74±0.51a
Petro AA	4	52.11±6.64c	60.48±0.08g
	8	41.38±0.72ab	62.89±0.07f
	12	66.72±1.28e	70.65±0.05b