Abstract:

Mycoplasma, as an important pathogen causing cattle infection, has been spread worldwide and can cause a series of serious diseases in cattle. Mycoplasma is highly contagious and difficult to eradicate, so rapid and accurate diagnostic methods are needed to prevent and control disease outbreaks. This article reviewed the development and application of different diagnostic methods for mycoplasma. The traditional identification and diagnosis of mycoplasma are mainly in isolation and culture. Recently, techniques for mycoplasma detection from samples using polymerase chain reaction (PCR) have increasingly matured. Compared with traditional diagnostic methods, PCR is more efficient, specific and sensitive. At present, pathogen isolation and identification, molecular biology identification, serological identification and other ways can be used for mycoplasma isolates typing, and easy to determine the gene characteristics in the disease outbreak investigation. Serological diagnosis by indirect ELISA can detect anti-mycoplasma antibodies in serum and milk, and has been demonstrated on single animal samples and BTM samples. The comprehensive application of these detection methods will contribute to the accurate detection of pathogens in production practice and the scientific assessment of the disease occurrence status of cattle herds.

Key words: Mycoplasma bovis, ELISA, mastitis, PCR, clinical diagnosis