Study on Determination Method of Pigment Content of Pear Peel

doi:10.11924/j.issn.1000-6850.casb2022-0786

Abstract

Abstract:

The paper aims to find a method suitable for determining the color pigment content of different pear peels. The chlorophyll content and total carotenoid content were determined for five pear varieties with different peel colors, namely ‘Xinli No.7’, ‘Akizuki’, ‘Yali’, ‘Yuluxiang’ and ‘Xianghongli’ by two methods. The results showed that for ‘Xinli No.7’ and ‘Yuluxiang’, the total chlorophyll content, chlorophyll a content, chlorophyll b content and total carotenoid content measured by the 80% acetone extraction method and the 95% ethanol extraction method weren’t significantly different. For ‘Yali’, ‘Xianghongli’ and ‘Akizuki’, the total carotenoid content measured by the 80% acetone extraction method and the 95% ethanol extraction method wasn’t significantly different, but the total chlorophyll content, chlorophyll a content and chlorophyll b content measured by the 80% acetone extraction method were significantly higher than the content measured by the 95% ethanol extraction method. The chlorophyll content and total carotenoid content of pear peels of all varieties can be determined by 80% acetone extraction. When determining the chlorophyll and total carotenoids of pear peel with green color and green as the background color with red halo, the 95% ethanol extraction method can be preferred. If the total carotenoid content is only required, all peel colors can be extracted with 95% ethanol.

Key words: pear, pericarp, chlorophyll, carotenoids, test methods

WU Yueju, ZHANG Xiaojie, JIA Zhuolong, ZHOU Yixin, HUANG Ping, WANG Xiaoming, PENG Jianying. Study on Determination Method of Pigment Content of Pear Peel[J]. Chinese Agricultural Science Bulletin, 2023, 39(28): 119-125.

Figures/Tables 6

References 25

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品种	提取方法	总叶绿素	叶绿素a	叶绿素b	总类胡萝卜素
新梨7号	丙酮80%(v/v)	4.021±0.667a	2.758±0.510a	1.075±0.278a	0.890±0.134a
新梨7号	乙醇95%(v/v)	3.450±0.438a	2.389±0.123a	0.932±0.137a	0.851±0.141a
玉露香	丙酮80%(v/v)	3.095±0.409a	2.202±0.109a	0.894±0.300a	0.697±0.058a
玉露香	乙醇95%(v/v)	2.756±0.491a	2.230±0.071a	0.526±0.420a	1.004±0.204a
鸭梨	丙酮80%(v/v)	0.501±0.0131a	0.199±0.027a	0.354±0.031a	0.190±0.014a
鸭梨	乙醇95%(v/v)	0.260±0.014b	0.098±0.016b	0.193±0.038b	0.145±0.011a
香红梨	丙酮80%(v/v)	2.399±0.239a	0.978±0.110a	1.412±0.144a	0.728±0.069a
香红梨	乙醇95%(v/v)	1.192±0.268b	0.502±0.071b	0.681±0.183b	0.591±0.039a
秋月	丙酮80%(v/v)	0.820±0.026a	0.291±0.004a	0.530±0.030a	0.131±0.019a
秋月	乙醇95%(v/v)	0.083±0.019b	0.037±0.075b	0.066±0.014b	0.096±0.094a

品种	提取方法	总叶绿素	叶绿素a	叶绿素b	总类胡萝卜素
新梨7号	丙酮80%(v/v)	4.021±0.667a	2.758±0.510a	1.075±0.278a	0.890±0.134a
新梨7号	乙醇95%(v/v)	3.450±0.438a	2.389±0.123a	0.932±0.137a	0.851±0.141a
玉露香	丙酮80%(v/v)	3.095±0.409a	2.202±0.109a	0.894±0.300a	0.697±0.058a
玉露香	乙醇95%(v/v)	2.756±0.491a	2.230±0.071a	0.526±0.420a	1.004±0.204a
鸭梨	丙酮80%(v/v)	0.501±0.0131a	0.199±0.027a	0.354±0.031a	0.190±0.014a
鸭梨	乙醇95%(v/v)	0.260±0.014b	0.098±0.016b	0.193±0.038b	0.145±0.011a
香红梨	丙酮80%(v/v)	2.399±0.239a	0.978±0.110a	1.412±0.144a	0.728±0.069a
香红梨	乙醇95%(v/v)	1.192±0.268b	0.502±0.071b	0.681±0.183b	0.591±0.039a
秋月	丙酮80%(v/v)	0.820±0.026a	0.291±0.004a	0.530±0.030a	0.131±0.019a
秋月	乙醇95%(v/v)	0.083±0.019b	0.037±0.075b	0.066±0.014b	0.096±0.094a