Physiological Response of Rubus idaeus Stems to Cryogenic Stress

doi:10.11924/j.issn.1000-6850.casb2022-0933

Abstract

Abstract:

The paper aims to explore the reasons for the difference in cold resistance between wild Rubus idaeus resources with low temperature resistance and low temperature sensitive R. idaeus cultivars, and to provide theoretical basis for breeding cold-resistant varieties. At the same time, principal component analysis was used to evaluate the cold resistance. The primary stems of wild R. idaeus resources R. crataegifolius 1, R. crataegifolius 2, and cultivars of R. idaeus of ‘Polana’, ‘Fertodi’, and ‘Heritage’ were used as experimental materials to explore the differences in relative water content, osmotic adjustment substances, antioxidant enzyme activity and membrane permeability during natural overwintering. The relative water content of the stem of the R. crataegifolius did not change much during the natural overwintering process, and reached the lowest value of 47.77% on January 11, 2021, while the relative water content of the stem of the R. idaeus cultivar decreased rapidly during the low temperature period (January 11), and the lowest value was only 12.83%. The SOD activity of R. idaeus stems fluctuated during the overwintering period, and the SOD activity of wild resources was significantly higher than that of cultivated varieties during most overwintering periods. The soluble protein content and proline content of R. crataegifolius stems were significantly higher than those of R. idaeus cultivars during the overwintering period, and the relative conductivity and MDA content of stems were significantly lower than those of R. idaeus cultivars. The cold resistance from strong to weak was R. crataegifolius 2, R. crataegifolius 1, ‘Polana’, ‘Fertodi’, and ‘Heritage’. The cold resistance of wild R. idaeus resources is significantly better than that of R. idaeus cultivars, mainly due to the strong water retention capacity of stems, high resistance to membrane lipid peroxidation, and the role of high osmotic regulatory substances and antioxidant enzyme systems under low temperature stress.

Key words: Rubus idaeus, stem, low temperature stress, physiological characteristics, cold resistance

WANG Xinyue, ZHANG Liye, ZHANG Xuemei. Physiological Response of Rubus idaeus Stems to Cryogenic Stress[J]. Chinese Agricultural Science Bulletin, 2023, 39(34): 63-72.

Figures/Tables 9

References 38

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月份	平均气温/℃	平均湿度/%
10月	14	47
11月	5.5	46
12月	-1.5	42
1月	-4	42
2月	0	42
3月	8	32

月份	平均气温/℃	平均湿度/%
10月	14	47
11月	5.5	46
12月	-1.5	42
1月	-4	42
2月	0	42
3月	8	32

日期	当日最高气温	当日最低气温	当日平均气温
2020-10-17	23	5	14
2020-11-04	16	2	9
2020-11-23	7	-3	2
2020-12-08	3	-6	-1.5
2020-12-26	5	-5	0
2021-01-11	2	-11	-4.5
2021-01-28	2	-10	-4
2021-02-18	10	-4	3
2021-03-07	9	-1	4
2021-03-25	22	10	16

日期	当日最高气温	当日最低气温	当日平均气温
2020-10-17	23	5	14
2020-11-04	16	2	9
2020-11-23	7	-3	2
2020-12-08	3	-6	-1.5
2020-12-26	5	-5	0
2021-01-11	2	-11	-4.5
2021-01-28	2	-10	-4
2021-02-18	10	-4	3
2021-03-07	9	-1	4
2021-03-25	22	10	16

	相对含水量	相对电导率	脯氨酸	可溶性蛋白	可溶性糖	淀粉	MDA	SOD	POD	CAT
相对含水量	1
相对电导率	-0.897^*	1
脯氨酸	0.992^**	-0.866	1
可溶性蛋白	0.803	-0.878	0.826	1
可溶性糖	-0.372	0.184	-0.459	-0.578	1
淀粉	-0.257	-0.146	-0.301	-0.014	0.665	1
MDA	-0.654	0.917^*	-0.600	-0.781	-0.002	0.426	1
SOD	0.984^**	-0.858	0.978^**	0.729	-0.279	-0.220	-0.589	1
POD	-0.984^**	0.932^*	-0.983^**	-0.895^*	0.444	0.193	0.726	-0.949^*	1
CAT	-0.591	0.335	-0.601	-0.131	-0.081	0.214	0.003	-0.720	0.473	1