Screening, Identification and Control Effect of Biocontrol Bacteria Against Kiwifruit Black Spot Disease

doi:10.11924/j.issn.1000-6850.casb2023-0437

Abstract

Abstract:

In order to screen the antagonistic bacteria against Diaporthe eres, strain BKH with good antagonistic effect was isolated from the rhizosphere soil of Actinidia chinensis in Zhouzhi County, Shaanxi Province by plate confrontation method. BKH strain was identified as Burkholderia ambifaria, through the methods of colony and microscopic morphology observation, physiological and biochemical test and 16S rDNA gene sequences analysis. BKH strain could significantly inhibit the growth of Diaporthe eres on the plate, and the inhibition rate was 72.5%. The field control effect of BKH strain against kiwifruit black spot disease was 91.7%, which had great potential in biological control of kiwifruit black spot disease.

Key words: kiwifruit black spot disease, Burkholderia ambifaria, biocontrol bacteria, identification, control effect

WANG Jiazhe, ZHANG Feng, LI Yingmei, ZHOU Bohang, FU Bo. Screening, Identification and Control Effect of Biocontrol Bacteria Against Kiwifruit Black Spot Disease[J]. Chinese Agricultural Science Bulletin, 2024, 40(13): 109-112.

Figures/Tables 6

References 19

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级值	病斑面积占果实总面积/%
0	0
1	(0，10)
3	[10，30)
5	[30，50)
7	[50，70)
9	[70，100]

级值	病斑面积占果实总面积/%
0	0
1	(0，10)
3	[10，30)
5	[30，50)
7	[50，70)
9	[70，100]

生化项目	结果
过氧化氢酶	+
脲酶	-
吲哚试验	-
硝酸盐还原试验	-
D-葡萄糖	+
L-阿拉伯糖	+
D-甘露糖	+
柠檬酸盐利用试验	+
麦芽糖	-

生化项目	结果
过氧化氢酶	+
脲酶	-
吲哚试验	-
硝酸盐还原试验	-
D-葡萄糖	+
L-阿拉伯糖	+
D-甘露糖	+
柠檬酸盐利用试验	+
麦芽糖	-

处理组	发病率/%	病情指数	防治效果/%
氨基酸钙叶面喷施	14.2b	5.67	64.8c
氨基酸钙根施	4.2c	3.27	79.6b
吡唑醚菌酯	1.8d	1.75	89.1a
菌株BKH	1.3d	1.33	91.7a
空白对照	25.6a	16