The Prevention and Control Effect of Ethanol Extract from Agrimonia pilosa on Highly Pathogenic Vibrio Infection in Litopenaeus vannamei

doi:10.11924/j.issn.1000-6850.casb2023-0800

Abstract

Abstract:

In order to investigate the preventive and therapeutic effects of the alcohol extract of Herba Agrimoniae on highly pathogenic Vibrio, this study analyzed the gut microbial, immune related genes and intestinal tissue of Litopenaeus vannamei infected with highly pathogenic Vibrio after feeding with 90% alcohol extract of Herba Agrimoniae for 3, 6, and 9 days. The results showed that the variety and number of bacteria in the intestines of sick shrimp increased. Among them, the number of pathogenic bacteria such as Shewanella, Vibrio and Spongiimonas were significantly higher than that of healthy shrimp, and the composition of the flora was significantly different from that of healthy shrimp. The relative abundance of the above pathogenic bacteria groups decreased significantly, while Lysobacter, a beneficial bacteria group, increased significantly. In addition, the alcohol extract of Herba Agrimoniae can increase the expression of immune related genes TLR, ALF, Crustin, and HSP70 in shrimp, protecting the intestinal tissue of shrimp. It was concluded that the addition of alcohol extract from Agrimonia pilosa to the feed could significantly improve the immunity of shrimp after infection with highly pathogenic Vibrio and enhance the antibacterial ability of shrimp.

Key words: Litopenaeus vannamei, gut microbes, immune genes, alcohol extract of herba agrimoniae, highly pathogenic vibrio, immunity, antibacterial capability

LIAO Sheguo, LAI Fuxiang, LIU Didi, ZHENG Hongchao, ZHENG Ran, LIN Kangqi, FAN Lanfen. The Prevention and Control Effect of Ethanol Extract from Agrimonia pilosa on Highly Pathogenic Vibrio Infection in Litopenaeus vannamei[J]. Chinese Agricultural Science Bulletin, 2024, 40(23): 153-164.

Figures/Tables 8

References 37

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基因	核酸序列(5′-3′)	GenBank登录号
Crustin	F：AACCAGAGACACCTGTTGGC	AY48497.1
Crustin	R：AGTCACCTCCTTCGGCTCTC	AY48497.1
ALF	F：TGTTCCTGGTGGCACTCTTC	GQ227486.1
ALF	R：GTCTCCTCGTTCCTCCACAG	GQ227486.1
TLR	F：TGCCAAGCAGTGATGTGA	KC660103.1
TLR	R：GCGGGAAGGAAGTGATGT	KC660103.1
HSP70	F：CCTCCAGGACTTCTTCAACG	EF495128
HSP70	R：GGTCACGTCCAACAGCAAC	EF495128
β-actin	F：GACTACCTGATGAAGATCC	AF300705
β-actin	R：TCGTTGCCGATGGTGATCA	AF300705

基因	核酸序列(5′-3′)	GenBank登录号
Crustin	F：AACCAGAGACACCTGTTGGC	AY48497.1
Crustin	R：AGTCACCTCCTTCGGCTCTC	AY48497.1
ALF	F：TGTTCCTGGTGGCACTCTTC	GQ227486.1
ALF	R：GTCTCCTCGTTCCTCCACAG	GQ227486.1
TLR	F：TGCCAAGCAGTGATGTGA	KC660103.1
TLR	R：GCGGGAAGGAAGTGATGT	KC660103.1
HSP70	F：CCTCCAGGACTTCTTCAACG	EF495128
HSP70	R：GGTCACGTCCAACAGCAAC	EF495128
β-actin	F：GACTACCTGATGAAGATCC	AF300705
β-actin	R：TCGTTGCCGATGGTGATCA	AF300705