Screening and Composting Application of Nitrogen-efficient Transformation Bacteria

doi:10.11924/j.issn.1000-6850.casb2024-0240

Abstract

Abstract:

The aim of this study is to screen nitrogen efficient transformation strains and verify their effects in the process of chicken manure composting, and to provide strain resources and theoretical support for the utilization and development of livestock and poultry manure in China. In this experiment, chicken manure and soil samples collected from different areas were used as the test materials, and the strains were isolated and purified by spread plate method. The nitrogen transformation ability of the strain was detected by Nessler's reagent colorimetric method and ultraviolet spectrophotometry. Then the microbial agent was prepared and applied to composting reaction. The results showed that 1 nitrogen efficient transformation bacterium was isolated and identified as Brucella tritici NC14. Biochar was selected as the best carrier to prepare microbial agent and then applied to compost. In the experiment of chicken manure composting, the application of microbial agent can effectively inhibit the release of NH₃ by 42.3%, reduce the content of NH₄⁺-N by 39.78% and increase the content of NO₃^--N by 52.3%. The results showed that strain NC14 had high nitrogen transformation ability and good application prospect.

Key words: nitrogen transformation, chicken manure compost, microbial agent, heterotrophic nitrification, screening

XU Xinyue, DAI Wenlong, FU Haiyan, LIU Chunguang, SONG Xinyu, YANG Fengshan. Screening and Composting Application of Nitrogen-efficient Transformation Bacteria[J]. Chinese Agricultural Science Bulletin, 2024, 40(32): 99-106.

Figures/Tables 9

References 34

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时间/h	氨氮降解率/%
时间/h	NC25	JC12	HC42	NC14
24	57.12±1.03b	47.79±0.45c	43.71±0.99d	67.22±0.91a
48	57.49±0.80b	49.53±0.34c	46.54±0.61d	70.43±0.91a
72	59.52±0.99b	49.71±0.33c	47.72±1.04d	71.78±0.78a

时间/h	氨氮降解率/%
时间/h	NC25	JC12	HC42	NC14
24	57.12±1.03b	47.79±0.45c	43.71±0.99d	67.22±0.91a
48	57.49±0.80b	49.53±0.34c	46.54±0.61d	70.43±0.91a
72	59.52±0.99b	49.71±0.33c	47.72±1.04d	71.78±0.78a

时间/h	硝态氮含量/(mg/L)
时间/h	NC25	JC12	HC42	NC14
24	1.69±1.07b	1.71±0.96c	1.30±1.18c	3.21±0.86a
48	1.78±0.89c	1.79±0.89c	1.87±1.66b	3.95±1.39a
72	1.83±1.49c	1.81±2.25c	1.92±1.06b	4.20±1.09a

时间/h	硝态氮含量/(mg/L)
时间/h	NC25	JC12	HC42	NC14
24	1.69±1.07b	1.71±0.96c	1.30±1.18c	3.21±0.86a
48	1.78±0.89c	1.79±0.89c	1.87±1.66b	3.95±1.39a
72	1.83±1.49c	1.81±2.25c	1.92±1.06b	4.20±1.09a

生理生化反应	NC14	生理生化反应	NC14
硝酸盐还原	+	V-P试验	-
氧化酶	+	葡萄糖分解	+
硫化氢	-	接触酶	+
甲基红试验	-	尿素	+
吲哚试验	-	柠檬酸盐利用	-