Effects of Melatonin on Seed Germination and Antioxidant Physiology of Foxtail Millet Seedlings Under Saline-alkali Stress

doi:10.11924/j.issn.1000-6850.casb2024-0537

Abstract

Abstract:

The study aimed to explore the effects of exogenous melatonin on seed germination and antioxidant physiology of foxtail millet seedlings under saline-alkali stress. Using the foxtail millet variety ‘Jigu 22’ as the experimental material, seeds were soaked in melatonin solutions at concentrations of 0, 100, 200, and 300 μmol/L. Germination index under saline-alkali stress was measured, and based on the germination test results, the melatonin concentration for seed soaking was selected for leaf spraying application during the seedling stage. At the seedling stage, a mixed saline-alkali solution (100 mmol/L, NaCl:NaHCO₃=4:1) was used to simulate saline-alkali stress. Seedling height, fresh weight, dry weight, malondialdehyde (MDA) and soluble sugar (SS) contents were measured, as well as the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). The results showed that saline-alkali stress significantly reduced germination vigor, germination rate, germination index, root length, shoot length, seedling height, and stems biomass accumulation in foxtail millet, while increasing the activities of SOD, POD, and CAT, as well as the contents of MDA and SS. Application of different concentrations of melatonin alleviated the adverse effects of saline-alkali stress on foxtail millet germination and seedling growth, among which the 200 μmol/L treatment exhibited the strongest protective effect. The application of the optimal melatonin concentration significantly enhanced the activities of antioxidant enzymes (SOD, POD, and CAT) and SS content while reducing MDA content. In summary, saline-alkali stress inhibits foxtail millet germination and seedling growth, whereas the application of an appropriate concentration of melatonin effectively mitigates the damage caused by saline-alkali stress and improves the salt-alkali tolerance of foxtail millet.

Key words: foxtail millet, saline-alkali stress, melatonin, germination stage, seedling stage, physiological index

ZHANG Mengyuan, WANG Siyu, XU Feng, LIU Yizhou, GUAN Yan’an, CHEN Erying, LI Feifei, YANG Yanbing, QIN Ling. Effects of Melatonin on Seed Germination and Antioxidant Physiology of Foxtail Millet Seedlings Under Saline-alkali Stress[J]. Chinese Agricultural Science Bulletin, 2025, 41(20): 60-66.

Figures/Tables 7

References 29

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代号		处理
M0+CK	0 μmol/L褪黑素浸种+蒸馏水
M1+CK	100 μmol/L褪黑素浸种+蒸馏水
M2+CK	200 μmol/L褪黑素浸种+蒸馏水
M3+CK	300 μmol/L褪黑素浸种+蒸馏水
M0+S	0 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫
M1+S	100 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫
M2+S	200 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫
M3+S	300 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫

代号		处理
M0+CK	0 μmol/L褪黑素浸种+蒸馏水
M1+CK	100 μmol/L褪黑素浸种+蒸馏水
M2+CK	200 μmol/L褪黑素浸种+蒸馏水
M3+CK	300 μmol/L褪黑素浸种+蒸馏水
M0+S	0 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫
M1+S	100 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫
M2+S	200 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫
M3+S	300 μmol/L褪黑素浸种+100 mmol/L混合盐碱溶液胁迫

代号	处理
MT0+CK	0 μmol/L褪黑素喷施+清水
MT200+CK	200 μmol/L褪黑素喷施+清水
MT0+S	0 μmol/L褪黑素喷施+100 mmol/L混合盐碱溶液胁迫
MT200+S	200 μmol/L褪黑素喷施+100 mmol/L混合盐碱溶液胁迫

代号	处理
MT0+CK	0 μmol/L褪黑素喷施+清水
MT200+CK	200 μmol/L褪黑素喷施+清水
MT0+S	0 μmol/L褪黑素喷施+100 mmol/L混合盐碱溶液胁迫
MT200+S	200 μmol/L褪黑素喷施+100 mmol/L混合盐碱溶液胁迫

处理	发芽势/%	发芽率/%	发芽指数	根长/cm	芽长/cm
M0+CK	88.67a	93.33a	91.38a	7.21a	1.96a
M1+CK	85.50a	92.00a	91.00ab	7.39a	2.03a
M2+CK	86.67a	92.00a	89.38c	7.61a	2.04a
M3+CK	88.00a	93.50a	92.17ab	7.58a	1.98a
M0+S	48.67c	74.00d	43.81e	1.23c	1.06c
M1+S	57.33b	76.67c	49.50d	1.52bc	1.28b
M2+S	59.33b	82.00b	52.25d	1.85b	1.31b
M3+S	56.00b	81.33b	50.31d	1.65bc	1.30b