Isolation and Identification of Pathogen of Luffa Wilt in Hunan Province and Evaluation of Resistant Germplasm

doi:10.11924/j.issn.1000-6850.casb2024-0620

Abstract

Abstract:

The paper aims to evaluate the wilt resistance of luffa germplasm and obtain resistant germplasm suitable for planting in Hunan. This study isolated the pathogen of luffa wilt disease from plants, and conducted morphological identification, molecular identification, and pathogenicity analysis of the pathogen. Then, using the established artificial inoculation identification technology system during the seedling stage, 126 luffa germplasms were inoculated and identified. The morphological identification results indicated that the pathogen had typical characteristics of Fusarium oxysporum. The amplification and sequencing of the internal transcribed spaces (ITS) sequence within the genome indicated that the pathogen was F. oxysporum and had strong pathogenicity. Using this strain to evaluate the disease resistance of the tested germplasm, 8 resistant germplasm, 17 moderately resistant germplasm, and 101 non resistant germplasm were obtained. This study provides technical support for in-depth exploration of resistance genes to luffa wilt disease, creation of resistant germplasm, and cultivation of new disease resistant varieties.

Key words: luffa, wilt, isolation and identification, Fusarium oxysporum, pathogenicity, artificial inoculation at seedling stage, resistant germplasm

HAN Xiaoxia, DONG Wenjing, MIN Ziyang, LI Yongqi, LU Ya’nan, YANG Jianhua, HU Xinjun, ZHU Hongjian. Isolation and Identification of Pathogen of Luffa Wilt in Hunan Province and Evaluation of Resistant Germplasm[J]. Chinese Agricultural Science Bulletin, 2025, 41(15): 117-123.

Figures/Tables 7

References 23

[1]	BAL K E, BAL Y, LALLAM A. Gross morphology and absorption capacity of cell-fibers from the fibrous vascular system of loofah (Luffa cylindrica)[J]. Textile research journal, 2004, 74(3):241-247.
[2]	田叶韩, 侯圆圆, 彭彩云, 等. 瓜类枯萎病菌遗传多样性和亲缘关系的SRAP分析[J]. 应用生态学报, 2017, 28(3):947-956. doi: 10.13287/j.1001-9332.201703.003
[3]	罗方芳, 何自福, 虞皓, 等. 广东丝瓜主要品种对枯萎病抗性的鉴定与评价[J]. 广东农业科学, 2009(12):49-50.
[4]	林永胜, 张武君, 周先治, 等. 43个丝瓜种质资源枯萎病抗性的鉴定与评价[J]. 福建农业科技, 2021, 28(5):28-32.
[5]	柴丹, 韩帅, 郭江洪, 等. 不同丝瓜种质资源对枯萎病的抗性鉴定与评价[J]. 长江蔬菜, 2021(22):71-73.
[6]	康玉妹, 薛珠政, 李永平, 等. 丝瓜新品种(系)对枯萎病抗性的鉴定与评价[J]. 农业科技通讯, 2022(8):126-129.
[7]	SCHMIDT O, MORETH U. Data bank of rDNA-ITS sequences from building-rot fungi for their identification[J]. Wood science & technology, 2002, 36(5):429-433.
[8]	韩小霞, 闵子扬, 李勇奇, 等. 利用简便SSR法鉴定‘兴蔬早佳’丝瓜杂交种纯度[J]. 中国农学通报, 2023, 39(31):44-49. doi: 10.11924/j.issn.1000-6850.casb2022-0549
[9]	林永胜, 张玉灿, 李祖亮, 等. 苦瓜砧木丝瓜新品种‘银砧1号’的选育[J]. 福建农业学报, 2015, 30(7):667-669.
[10]	王平. 黄瓜高温闷棚及嫁接有关的栽培生理研究[D]. 南京: 南京农业大学, 2016.
[11]	欧阳昌东. 丝瓜作砧木嫁接对‘科农二号’苦瓜生长,产量,抗性和品质的影响[D]. 南昌: 江西农业大学, 2017.
[12]	先本刚, 詹成波, 张建军. 苦瓜专用砧木丝瓜新品种蓉砧1号的选育[J]. 长江蔬菜, 2016(6):12-14.
[13]	郭江洪, 帅正彬, 柴丹苦, 等. 苦瓜专用砧木丝瓜新品种‘蓉砧3号’[J]. 园艺学报, 2024, 51(S1):113-114.
[14]	陈位平, 蒋万, 张忠武, 等. 湖南夏秋苦瓜嫁接砧木品种筛选[J]. 安徽农业科学, 2024, 52(5):50-53.
[15]	林永胜, 陈阳. 不同丝瓜砧木嫁接对苦瓜产量品质及抗病性的影响[J]. 中国蔬菜, 2024(7):86-91.
[16]	周红梅, 毛爱军, 张丽蓉, 等. 黄瓜枯萎病接种方法及抗性遗传的研究[J]. 华北农学报, 2010, 25(4):186-190. doi: 10.7668/hbnxb.2010.04.039
[17]	李晓慧, 梁慎, 常高正, 等. 西瓜枯萎病苗期抗性鉴定及抗源筛选[J]. 江苏农业科学, 2018, 46(17):91-94.
[18]	刘朋义, 别之龙, 彭斌, 等. 甜瓜品种抗枯萎病的苗期鉴定[J]. 中国瓜菜, 2011, 24(2):11-13.
[19]	周红梅, 董从娟, 张海英, 等. 黄瓜抗枯萎病基因连锁分析和定位[J]. 分子植物育种, 2015, 13(9):1980-1986.
[20]	张敬敬, 张海英, 潘秀清, 等. 河北省130份西瓜品种与种质资源抗病基因KASP检测分析[J]. 华北农学报, 2019, 50(1):131-141.
[21]	OUMOULOUD A, AMEDO-ANDRES M S, GONZALEZ-TOREE R, et al. Development of molecular markers linked to the Fom-1 locus for resistance to Fusarium race 2 in melon[J]. Euphytica, 2008, 164(2):347-356.
[22]	李娜, 王吉明, 尚建立, 等. 西瓜枯萎病生理小种1抗性QTL精细定位与InDel标记开发[J]. 中国农业科学, 2017, 50(1):131-141. doi: 10.3864/j.issn.0578-1752.2017.01.012
[23]	SOUSARAEI N, RAMSHINI N, LOTFI M, et al. Marker assisted backcrossing for introgression of Fusarium wilt resistance gene into melon[J]. Euphytica, 2018, 214(1):1-10.

序号	编号	发病率/%	病情指数	抗性等级	序号	编号	发病率/%	病情指数	抗性等级
1	H1	100	77.78	HS	24	H95	100	76.39	HS
2	H4	100	84.44	HS	25	H96	100	39.68	MR
3	H3	100	18.52	R	26	H43	100	47.47	MR
4	H13	100	86.11	HS	27	H44	100	88.89	HS
5	H15	100	89.90	HS	28	H104	100	38.89	MR
6	H24	100	41.41	MR	29	H105	100	59.72	S
7	H25	100	55.56	S	30	H106	100	51.28	MR
8	H26	100	25.93	R	31	H107	100	83.33	HS
9	H29	100	90.13	HS	32	H109	100	56.48	S
10	H38	100	45.45	MR	33	H112	100	30.10	MR
11	H39	100	74.60	S	34	H113	100	35.35	MR
12	H30	100	40.74	MR	35	H115	100	51.85	MR
13	H31	100	57.58	S	36	H117	100	96.30	HS
14	H40	100	20.00	R	37	H118	100	47.47	MR
15	H45	100	95.56	HS	38	H121	100	24.44	R
16	H46	100	37.37	MR	39	H122	100	44.44	MR
17	H60	100	30.16	MR	40	H126	100	52.38	MR
18	H61	100	81.82	HS	41	H127	100	55.56	S
19	H86	100	25.93	R	42	H128	100	22.22	R
20	H88	100	42.22	MR	43	H129	100	73.74	S
21	H90	100	63.33	S	44	H130	100	33.33	R
22	H91	100	55.56	S	45	H131	100	52.78	MR
23	H93	100	92.59	HS	46	H293	100	53.09	MR

序号	编号	发病率/%	病情指数	抗性等级	序号	编号	发病率/%	病情指数	抗性等级
1	H1	100	77.78	HS	24	H95	100	76.39	HS
2	H4	100	84.44	HS	25	H96	100	39.68	MR
3	H3	100	18.52	R	26	H43	100	47.47	MR
4	H13	100	86.11	HS	27	H44	100	88.89	HS
5	H15	100	89.90	HS	28	H104	100	38.89	MR
6	H24	100	41.41	MR	29	H105	100	59.72	S
7	H25	100	55.56	S	30	H106	100	51.28	MR
8	H26	100	25.93	R	31	H107	100	83.33	HS
9	H29	100	90.13	HS	32	H109	100	56.48	S
10	H38	100	45.45	MR	33	H112	100	30.10	MR
11	H39	100	74.60	S	34	H113	100	35.35	MR
12	H30	100	40.74	MR	35	H115	100	51.85	MR
13	H31	100	57.58	S	36	H117	100	96.30	HS
14	H40	100	20.00	R	37	H118	100	47.47	MR
15	H45	100	95.56	HS	38	H121	100	24.44	R
16	H46	100	37.37	MR	39	H122	100	44.44	MR
17	H60	100	30.16	MR	40	H126	100	52.38	MR
18	H61	100	81.82	HS	41	H127	100	55.56	S
19	H86	100	25.93	R	42	H128	100	22.22	R
20	H88	100	42.22	MR	43	H129	100	73.74	S
21	H90	100	63.33	S	44	H130	100	33.33	R
22	H91	100	55.56	S	45	H131	100	52.78	MR
23	H93	100	92.59	HS	46	H293	100	53.09	MR