Antimicrobial Activity of Extracts from Stellera chamaejasme Against Three Vegetable Pathogenic Fungi

doi:10.11924/j.issn.1000-6850.casb2023-0530

Abstract

Abstract:

To develop botanical fungicide that can inhibit vegetable pathogens in protected cultivation, anhydrous ethanol extracts of Stellera chamaejasme with different concentration gradients were used to study the inhibitory activity of Rhizoctonia solani, Fusarium oxysporum and Gibellulopsis nigrescens by mycelial growth rate method, and the virulence was analyzed. The results showed that different concentrations of S. chamaejasme extracts had different degrees of inhibitory effect on three vegetable pathogens, and the inhibitory effect was enhanced with the increase of extract concentration. When the concentration of the extract were 8 mg/mL and 10 mg/mL, the inhibition rate of R. solani and F. oxysporum could reach more than 50%, and when the concentration of the extract was 15 mg/mL, the inhibition rate was the highest, which was 87.38% and 92.84%, respectively. Only when the concentration of the extract reached 20 mg/mL, the inhibition rate of the extract against G. nigrescens could reach over 50% (59.09%). The EC₅₀ values of effective medium concentration were as follows: G. nigrescens (15.64 mg/mL)> F. oxysporum (9.08 mg/mL)> R. solani (5.62 mg/mL). Therefore, the good antibacterial effect of extracts of S. chamaejasme is proven on all the three pathogenic fungi tested, among which the inhibitory effect on R. solani is the strongest, and that on G. nigrescens is the weakest.

Key words: Stellera chamaejasme, vegetables in green house, plant extract, fusarium wilt, antibacterial effect

SUN Jing, CHENG Lixin, LI Yunhua, YUE Linfang, LIU Fang, PANG Yajuan. Antimicrobial Activity of Extracts from Stellera chamaejasme Against Three Vegetable Pathogenic Fungi[J]. Chinese Agricultural Science Bulletin, 2024, 40(19): 115-119.

Figures/Tables 5

References 26

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提取液浓度/(mg/mL)	菌落直径/cm	抑菌率/%
0(CK)	8.00±0.00a	0.00±0.00f
3	5.65±0.27b	32.24±3.70e
5	4.98±0.15c	41.39±2.00d
8	3.71±0.24d	58.72±3.27c
10	3.11±0.37e	66.99±5.10b
15	1.62±0.27f	87.38±3.68a

提取液浓度/(mg/mL)	菌落直径/cm	抑菌率/%
0(CK)	8.00±0.00a	0.00±0.00f
3	5.65±0.27b	32.24±3.70e
5	4.98±0.15c	41.39±2.00d
8	3.71±0.24d	58.72±3.27c
10	3.11±0.37e	66.99±5.10b
15	1.62±0.27f	87.38±3.68a

提取液浓度/(mg/mL)	菌落直径/cm	抑菌率/%
0(CK)	7.65±0.26a	0.00±3.79e
3	7.47±0.18a	2.67±2.52e
5	6.39±0.31b	18.10±4.48d
8	5.55±0.45c	30.21±6.50c
10	3.86±0.50d	54.56±7.19b
15	1.20±0.11e	92.84±1.63a

提取液浓度/(mg/mL)	菌落直径/cm	抑菌率/%
0(CK)	7.65±0.26a	0.00±3.79e
3	7.47±0.18a	2.67±2.52e
5	6.39±0.31b	18.10±4.48d
8	5.55±0.45c	30.21±6.50c
10	3.86±0.50d	54.56±7.19b
15	1.20±0.11e	92.84±1.63a

提取液浓度/(mg/mL)	菌落直径/cm	抑菌率/%
0(CK)	5.59±0.18f	0.00±3.72f
3	5.44±0.13f	2.93±2.70f
5	5.04±0.25e	11.17±5.18e
8	4.21±0.23d	28.10±4.70d
10	3.94±0.16c	33.68±3.24c
15	3.43±0.21b	44.10±4.28b
20	2.70±0.52a	59.09±10.66a