Interaction Between Plant Growth Regulator and Fungicide to Control Cotton Fusarium wilt and the Synergy Mechanism

doi:10.11924/j.issn.1000-6850.casb2022-0576

Abstract

Abstract:

We designed this study to explore the combination of plant growth regulator and fungicide, which could benefit the cotton resistance to Fusarium wilt. Moreover, the synergy mechanism of the combination was investigated, to provide insights into enhancing cotton resistance to Fusarium wilt. Through indoor toxicity test, two fungicides with the minimum EC₅₀ were selected. The most suitable plant growth regulators were also identified by seed vigor testing and conidial germination statistic of Fusarium wilt. The combination of fungicide and plant growth regulator was applied during the plant growth in greenhouse to evaluate the synergy effect. To explore the mechanism underlying the combination of fungicide and plant growth regulator, we detected the expression levels of genes involving in jasmonic acid-related and salicylic acid-related pathways by qRT-PCR. The results of fungicide selection showed that carbendazim and carboxin had the minimum EC₅₀ and the strongest virulence to Fusarium wilt, the EC₅₀ was 13.894 mg/L and 29.566 mg/L, respectively. Plant growth regulator selection indicated that the optimal concentration 6-Benzylaminopurine and naphthylacetic acid was 5 mg/L and 50 mg/L, respectively. Finally, the most suitable combination was 6-Benzylaminopurine-carbendazim, which had the control effect of 80.95% and significant synergy effect. The results of qRT-PCR also showed that genes in jasmonic acid-related and salicylic acid-related pathways had significantly higher expression levels in the treated group than those in blank control group. In conclusion, the 6-Benzylaminopurine-carbendazim combination could enhance the cotton resistance to Fusarium wilt by activating transcription of genes in jasmonic acid-related and salicylic acid-related pathways.

Key words: cotton Fusarium wilt, plant growth regulator, fungicide, interaction, mechanism

ZHENG Deyou, ZUO Dongyun, WANG Qiaolian, LV Limin, CHENG Hailiang, GU Aixing, SONG Guoli. Interaction Between Plant Growth Regulator and Fungicide to Control Cotton Fusarium wilt and the Synergy Mechanism[J]. Chinese Agricultural Science Bulletin, 2023, 39(18): 136-141.

Figures/Tables 9

References 25

[1]	李海薇, 韩万里, 王梦瑶, 等. 棉花枯萎病拮抗短小芽胞杆菌筛选鉴定及生防研究[J]. 中国生物防治学报, 2018, 34(3):440-448. doi: 10.16409/j.cnki.2095-039x.2018.03.016
[2]	高文章, 韩新才, 代文哲, 等. 新型苯并咪唑类化合物对棉花枯萎病菌的毒力测定[J]. 棉花科学, 2020, 42(4):40-44.
[3]	周建刚, 赵永红, 王惠滨. 几种药剂对棉花枯萎病防治效果[J]. 中国棉花, 2004(7):23.
[4]	杨兴洪, 陈翠容, 施培. 植物生长调节剂在棉花上的研究应用概况及展望[J]. 山东农业大学学报, 1996(2):236-240.
[5]	李田甜, 陈国栋, 万素梅, 等. 叶面喷施不同配方植物生长调节剂对棉花苗期根系生长的影响[J]. 山东农业科学, 2022, 54(2):46-50.
[6]	PENNUCCI A, JACKSON N. Disstinguisning phytotoxictty and pathogenicity by funci in growth retardant treated turfgrass[C]. Phytopathology, 1986, 76(6):657-657.
[7]	霍成君, 韩建国, 毛培胜, 等. 矮壮素和多效唑对草地早熟禾草坪质量的影响[J]. 草地学报, 2000(2):137-143. doi: 10.11733/j.issn.1007-0435.2000.02.010
[8]	朱荷琴, 冯自力, 师勇强, 等. 利用植物疫苗及生长调节剂缩节胺控制棉花黄萎病[J]. 中国棉花, 2010, 37(8):10-12.
[9]	蒋家珍, 姜世聚, 邱立红, 等. 油菜素内酯与丙环唑互作对棉苗病害的影响及机理研究[J]. 湖南农业科学, 2004(5):49-51.
[10]	陈晶, 魏朝霞, 唐嘉义. 3种生物农药对4种茶树病害的室内抑菌试验[J]. 云南农业大学学报(自然科学), 2012, 27(3):453-456.
[11]	檀根甲, 祝建平. 杀菌剂生物测定计算方法及应用[J]. 安徽农学通报, 1998(1):28-29.
[12]	孙君灵, 宋晓轩, 朱荷琴, 等. 杀菌剂对棉花种子活力的影响[J]. 种子, 2000(1):23-25.
[13]	周德庆, 徐士菊. 微生物学词典[M]. 天津: 天津科学技术出版社, 2005.
[14]	朱荷琴, 冯自力, 李志芳, 等. 蛭石沙土无底纸钵定量蘸菌液法鉴定棉花品种(系)的抗黄萎病性[J]. 中国棉花, 2010, 37(12):15-17.
[15]	周京龙, 冯自力, 魏锋, 等. 棉花内生细菌YUPP-10及其分泌蛋白CGTase对棉花枯萎病的防治作用及机理[J]. 中国农业科学, 2021, 54(17):3691-3701. doi: 10.3864/j.issn.0578-1752.2021.17.011
[16]	中华人民共和国农业部. GB/T 22101.4—2009,棉花抗病虫性评价技术规范第4部分:枯萎病[S]. 北京: 中国标准出版社, 2009.
[17]	MANSOUR N A, ELDEFRAWI M E, TOPPOZADA A, et al. Toxicological studies on the Egyptian cotton leaf worm, Prodenia litura. VI. potentiation and antagonism of organophosphorus and carbamate insecticides[J]. Journal of economic entomology, 1966, 59(2):307-311. doi: 10.1093/jee/59.2.307 URL
[18]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4):402-408. doi: 10.1006/meth.2001.1262 URL
[19]	马存. 棉花枯萎病和黄萎病的研究[M]. 北京: 中国农业出版社, 2007.
[20]	赵龙飞, 徐亚军, 邓振山, 等. 拮抗棉花枯萎病菌的地黄内生细菌筛选、鉴定和促生潜能[J]. 微生物学报, 2021, 61(8):2338-2357.
[21]	ZHANG Z X, DIAO H L, WANG H Y, et al. Use of Ganoderma lucidum polysaccharide to control cotton Fusarium wilt, and the mechanism involved[J]. Pesticide biochemistry and physiology, 2019, 158(3):149-155. doi: 10.1016/j.pestbp.2019.05.003 URL
[22]	袁晓娟, 尹旭格, 曹欣, 等. 含短小芽孢杆菌的种衣剂对棉花枯萎病防治效果的研究[J]. 新疆农业大学学报, 2019, 42(5):365-371.
[23]	王成菊, 李学峰, 李常平, 等. 油菜素内酯与杀菌剂互作对棉花苗期病害的影响[J]. 中国棉花, 2003(12):5-7.
[24]	王成菊, 李常平, 郑明奇, 等. 植物生长调节剂与杀菌剂互作对棉花立枯病的影响[J]. 农药, 2003(12):36-38.
[25]	马晖玲, 房媛媛. 植物抗病性及诱导抗性在匍匐翦股颖病害防治中的应用[J]. 草业学报, 2014, 23(5):312-320. doi: 10.11686/cyxb20140537

杀菌剂	有效成分含量（质量浓度）/%	剂型	生产厂家
甲基立枯磷	95	原药	宁夏三丰化工有限公司
萎锈灵	98	原药	河南金鹏化工有限公司
甲基硫菌灵	97	原药	河北赛丰生物科技有限公司
苯菌灵	95	原药	河北冠龙农化有限公司
多菌灵	98	原药	河北冠龙农化有限公司
萘乙酸	95	原药	鹤壁全丰生物科技有限公司
苄氨基嘌呤	99	原药	郑州先利达化工有限公司

杀菌剂	有效成分含量（质量浓度）/%	剂型	生产厂家
甲基立枯磷	95	原药	宁夏三丰化工有限公司
萎锈灵	98	原药	河南金鹏化工有限公司
甲基硫菌灵	97	原药	河北赛丰生物科技有限公司
苯菌灵	95	原药	河北冠龙农化有限公司
多菌灵	98	原药	河北冠龙农化有限公司
萘乙酸	95	原药	鹤壁全丰生物科技有限公司
苄氨基嘌呤	99	原药	郑州先利达化工有限公司

基因	引物名称	引物序列(5′→3′)
GhJAZ	GhJAZ-F	GGTTCCTCTAACGCCAACGGAG
GhJAZ	GhJAZ-R	AAGATCTTCCGATGGGACGGGT
GhMYC2	GhMYC2-F	TGAACCACGTAGAAGCAGAGCG
GhMYC2	GhMYC2-R	ATACGTTAGGAACCACGGCACG
GhLOX1	GhLOX1-F	ATGTTCCTGGTCGAGGTCCCAT
GhLOX1	GhLOX1-R	GGGAGCCTTCCCAAATCGTTGT
GhAOS	GhAOS-F	AATCATCTGGTTCCATTCAAG
GhAOS	GhAOS-R	CTCCGTTGCTAATCTATTGTG
GhNPR1	GhNPR1-F	ACCACGACTGAGTTGCTTGACC
GhNPR1	GhNPR1-R	ATACACAACCTGTCCTTCGGCG
GhTGA	GhTGA-F	GCTGCTCGTAAAAGTCGGTTGC
GhTGA	GhTGA-R	ACCCTGTTGCGTTACTCGTTGA
GhUBQ7	GhUBQ7-F	GGAATCCACTCTACACCTT
GhUBQ7	GhUBQ7-R	GCTTGACCTTCTTCTTCTTG

基因	引物名称	引物序列(5′→3′)
GhJAZ	GhJAZ-F	GGTTCCTCTAACGCCAACGGAG
GhJAZ	GhJAZ-R	AAGATCTTCCGATGGGACGGGT
GhMYC2	GhMYC2-F	TGAACCACGTAGAAGCAGAGCG
GhMYC2	GhMYC2-R	ATACGTTAGGAACCACGGCACG
GhLOX1	GhLOX1-F	ATGTTCCTGGTCGAGGTCCCAT
GhLOX1	GhLOX1-R	GGGAGCCTTCCCAAATCGTTGT
GhAOS	GhAOS-F	AATCATCTGGTTCCATTCAAG
GhAOS	GhAOS-R	CTCCGTTGCTAATCTATTGTG
GhNPR1	GhNPR1-F	ACCACGACTGAGTTGCTTGACC
GhNPR1	GhNPR1-R	ATACACAACCTGTCCTTCGGCG
GhTGA	GhTGA-F	GCTGCTCGTAAAAGTCGGTTGC
GhTGA	GhTGA-R	ACCCTGTTGCGTTACTCGTTGA
GhUBQ7	GhUBQ7-F	GGAATCCACTCTACACCTT
GhUBQ7	GhUBQ7-R	GCTTGACCTTCTTCTTCTTG

杀菌剂	毒力回归方程	EC₅₀/(mg/L)	决定系数R²
多菌灵	y=-1.089+0.953x	13.894	0.938
萎锈灵	y=-2.621+1.782x	29.566	0.931
苯菌灵	y=-1.417+0.892x	38.748	0.913
甲基立枯磷	y=-1.023+0.627x	42.655	0.914
甲基硫菌灵	y=-6.925+3.745x	70.612	0.934