Abstract:

In order to ensure the purity of ‘Xingshu Zaojia’ luffa, a set of safe, reliable, simple and fast hybrid purity identification methods should be established. In this research, we adopted the simplified SSR molecular marker technology, used the hybrid of ‘Xingshu Zaojia’ luffa and its parents as test materials, and screened a pair of primers that could stably amplify the hybrid and its parents from 50 pairs of SSR primers, and the amplification products were co-dominant. This pair of primers was used to identify the purity of ‘Xingshu Zaojia’ hybrids produced in different places, and the error between the simplified SSR and the field morphological identification results was less than 1.2%. A pair of primers suitable for identifying the purity of ‘Xingshu Zaojia’ hybrid was obtained, and a set of simple methods suitable for identifying the seed purity of this variety was established. Using this pair of primers to identify the seed purity of ‘Xingshu Zaojia’ luffa hybrid can not only save the identification cost, but also shorten the identification cycle, which can provide technical guarantee for the safe sale of the commercial variety.

Key words: luffa, ‘Xingshu Zaojia’, hybrid, SSR molecular markers, purity identification