Effects of Hybridization on Growth and Development and Detoxification Metabolic Enzyme Activity of Plutella xylostella Progenies

doi:10.11924/j.issn.1000-6850.casb2022-0815

Abstract

Abstract:

The research aims to clarify the effect of hybridization between susceptible and resistant diamondback moth (DBM) on the growth, development and resistance of its progeny, and to explore new methods for the resistance management of DBM. In this study, the susceptible population hybridized with field resistant population of Plutella xylostella. The growth and development, sensitivity to pesticides and detoxification metabolic enzyme activity in F₁ generation of diamondback moth were analyzed. The results showed that the hybridization of susceptible and resistant diamondback moth had no significant effect on the fecundity, developmental duration, pupation rate and emergence rate of Plutella xylostella. The sensitivity of DBM orthogonal and reverse cross populations F₁ to indoxacarb and spinetoram was improved. The resistant ratio of DBM orthogonal F₁ to indoxacarb and spinetoram decreased more obviously. Compared with the resistant population, the resistant ratio decreased from 27.47 or 41.00 times to 6.82 or 21.50 times, respectively. The activities of glutathione-s transferase (GSTs) in the hybrid offspring of diamondback moth decreased significantly. The enzyme activities of DBM orthogonal and reverse cross populations decreased by 67.29% and 53.80%, respectively. After hybridization between sensitive and resistant diamondback moth, the sensitivity of diamondback moth to pesticides may be affected by regulating the activity of metabolic enzymes. The results can provide a new idea for the management of diamondback moth resistance.

Key words: Plutella xylostella, hybridization, pesticides, resistance, metabolic enzyme

YIN Fei, GE Tiancheng, XIAO Yong, PENG Zhengke, LI Zhenyu. Effects of Hybridization on Growth and Development and Detoxification Metabolic Enzyme Activity of Plutella xylostella Progenies[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 126-132.

Figures/Tables 3

References 42

[1]	ZALUCKI M P, ASAD S, REHAN S, et al. Estimating the economic cost of one of the world's major insect pests, Plutella xylostella (Lepidoptera: Plutellidae): just how long is a piece of string?[J]. Journal of economic entomology, 2012(4):4.
[2]	徐巨龙, 李静静, 王念猛, 等. 我国部分地区田间小菜蛾种群对8种常用杀虫剂的抗性检测[J]. 植物保护, 2021, 47(2):239-242.
[3]	李振宇, 肖勇, 吴青君, 等. 小菜蛾种群灾变及抗药性治理研究进展[J]. 应用昆虫学报, 2020, 57(3):549-567.
[4]	LI Z, FENG X, LIU S S, et al. Biology, ecology, and management of the diamondback moth in China[J]. Annual review of entomology, 2015, 61(1):277-296. doi: 10.1146/ento.2016.61.issue-1 URL
[5]	FURLONG M J, WRIGH T. Diamondback moth ecology and management: Problems, progress, and prospects[J]. Annual review of entomology, 2013, 58:517. doi: 10.1146/annurev-ento-120811-153605 pmid: 23020617
[6]	ANKERSMIT G W. DDT-resistance in Plutella maculipennis (Curt.) (Lep.) in Java[J]. Bulletin of entomological research, 1953, 44(3):421. doi: 10.1017/S0007485300025530 URL
[7]	EZIAH V Y, ROSE H A, CLIFT A D, et al. Susceptibility of four field populations of the diamondback moth Plutella xylostella L. (Lepidoptera:Yponomeutidae) to six insecticides in the Sydney region, New South Wales, Australia[J]. Australian journal of entomology, 2010, 47(4):355-360. doi: 10.1111/j.1440-6055.2008.00668.x URL
[8]	XINGLIANG W, YIDONG W. High Levels of resistance to chlorantraniliprole evolved in field populations of Plutella xylostella[J]. Journal of economic entomology, 2012(3):1019-1023.
[9]	TAMILSELVAN R, KENNEDY J S, SUGANTHI A. Monitoring the resistance and baseline susceptibility of Plutella xylostella (L.) (Lepidoptera: Plutellidae) against spinetoram in Tamil Nadu, India[J]. Crop protection, 2020, 142:105491. doi: 10.1016/j.cropro.2020.105491 URL
[10]	刘喃喃, 朱芳, 徐强, 等. 昆虫抗药性机理:行为和生理改变及解毒代谢增强(英文)[J]. 昆虫学报, 2006(4):671-679.
[11]	黄云, 吴沛卓, 郑钧月, 等. 棉铃虫对化学杀虫剂的抗性现状及分子机制研究进展[J]. 植物保护学报, 2022, 49(1):336-350.
[12]	ZUO Y, SHI Y, ZHANG F, et al. Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm[J]. PLoS Genetics, 2021, 17:e1009680. doi: 10.1371/journal.pgen.1009680 URL
[13]	李欣莲, 齐易香, 陆永跃. 重大实蝇类昆虫解毒代谢基因的研究进展[J]. 植物保护学报, 2022, 49(1):351-365.
[14]	SONODA S, IGAKI C. Characterization of acephate resistance in the diamondback moth Plutella xylostella[J]. Pesticide biochemistry & physiology, 2010, 98(1):121-127.
[15]	尹飞, 陈焕瑜, 冯夏, 等. 乙基多杀菌素抗性小菜蛾代谢解毒酶酶活性研究[J]. 应用昆虫学报, 2016, 53(2):314-319.
[16]	齐浩亮, 崔丽, 王芹芹, 等. 溴虫氟苯双酰胺对小菜蛾的毒力及相关酶活性的影响[J]. 植物保护, 2017, 43(1):112-116.
[17]	申君, 鲁艳辉, 张淑真, 等. 亚致死浓度氰氟虫腙对小菜蛾三种解毒酶及P450 mRNA表达量的影响[J]. 应用昆虫学报, 2016, 53(2):256-263.
[18]	谢苗, 尤民生. 氟虫腈对小菜蛾幼虫体内解毒酶的影响[J]. 应用昆虫学报, 2011, 48(6):1728-1733.
[19]	尹飞, 冯夏, 李振宇, 等. 亚致死剂量氯虫苯甲酰胺对小菜蛾体内活性酶的影响[J]. 植物保护, 2014, 40(2):66-69.
[20]	席羽, 殷坤山, 肖强. 不同地理种群茶尺蠖对EoNPV的敏感性差异研究[J]. 茶叶科学, 2011, 31(2):100-104.
[21]	席羽, 殷坤山, 唐美君, 等. 浙江茶尺蠖地理种群已分化成为不同种[J]. 昆虫学报, 2014, 57(9):1117-1122.
[22]	张灿, AHMED MUHAMMAD Z, 马骏, 等. 桔小实蝇中国与马来西亚种群间杂交:融合性、生物学及检疫挑战(英文)[J]. 环境昆虫学报, 2018, 40(3):553-570.
[23]	谭荣鹤, 朱道弘, 阳艳萍. 中华稻蝗不同地理种群杂交子代的滞育率[J]. 昆虫知识, 2008(3):394-397.
[24]	党志红, 安静杰, 高占林, 等. 河北省不同地区棉蚜种群对6种杀虫剂抗药性与种群适合度[J]. 植物保护, 2019, 45(3):111-114.
[25]	齐素敏, 吴有芳, 李如美, 等. 山东省不同地区韭蛆种群对杀虫剂的抗药性[J]. 植物保护, 2016, 42(4):179-183.
[26]	李良德, 王定锋, 吴光远, 等. 福建省3个地区茶小绿叶蝉对5种常用农药的抗药性比较[J]. 茶叶学报, 2020, 61(3):133-137.
[27]	李仁, 梁平卓, 程沈航, 等. 我国棉蚜对吡虫啉和氟啶虫胺腈抗性水平监测与交互抗性分析[J]. 植物保护学报, 2021, 48(5):1104-1113.
[28]	刘佳, 周勇, 朱航, 等. 斜纹夜蛾抗药性监测及茚虫威对其解毒代谢酶的影响[J]. 昆虫学报, 2016, 59(11):1254-1262. doi: 10.16380/j.kcxb.2016.11.014
[29]	朱勋, 杨家强, 吴青君, 等. 小菜蛾不同地理种群遗传多样性的ISSR标记研究[J]. 昆虫学报, 2012, 55(8):981-987.
[30]	尹飞, 冯夏, 李振宇, 等. 不同地区小菜蛾对氯虫苯甲酰胺的抗药性差异[J]. 植物保护, 2015, 41(5):160-163.
[31]	王婷婷, 张春妮. 溴虫腈对小菜蛾幼虫解毒酶的影响[J]. 西北农业学报, 2014, 23(8):196-200.
[32]	周利琳, 司升云, 汪钟信, 等. 甜菜夜蛾对虫酰肼抗性的生化机制[J]. 昆虫学报, 2009, 52(4):386-394.
[33]	程英, 李凤良, 金剑雪, 等. 小菜蛾不同抗性品系及其杂交F1代的生物适合度[J]. 农药学学报, 2014, 16(1):49-53.
[34]	程英, 李凤良, 金剑雪, 等. 小菜蛾不同抗性品系与敏感品系杂交的生物适合度[J]. 西南农业学报, 2014, 27(5):1957-1960.
[35]	吴青君, 张文吉, 张友军, 等. 敏感和抗阿维菌素小菜蛾的生物适合度[J]. 农药学学报, 2000(1):36-40.
[36]	刘泽文, 韩召军, 张玲春. 褐飞虱不同品系杂交子代抗药性和适合度的变化[J]. 中国水稻科学, 2004(2):81-84.
[37]	冯紫姣. 桔小实蝇对马拉硫磷的抗性遗传方式及生化机理研究[D]. 重庆: 西南大学, 2015.
[38]	SILVER K S, SODERLUND D M. Action of pyrazoline-type insecticides at neuronal target sites[J]. Pesticide biochemistry & physiology, 2005, 81(2):136-143.
[39]	王芹芹, 崔丽, 王立, 等. 茚虫威对草地贪夜蛾的毒力及解毒酶的诱导作用[J]. 植物保护, 2020, 46(1):78-81.
[40]	王芹芹, 崔丽, 王奇渊, 等. 棉铃虫对茚虫威的抗性机理:PBO,DEF和DEM的增效作用及解毒酶活性[J]. 昆虫学报, 2017, 60(8):912-919. doi: 10.16380/j.kcxb.2017.08.008
[41]	王茹梦. PxGSTO4介导小菜蛾对茚虫威解毒代谢机制研究[D]. 武汉: 华中农业大学, 2019.
[42]	田雪莲, 尹显慧, 龙友华, 等. 低剂量乙基多杀菌素对小菜蛾解毒酶的影响[J]. 农药学学报, 2016, 18(5):589-595.

种群	药剂	斜率±标准误	LC₅₀(95%置信区间)/(mg/L)	X²(df)	抗性倍数
R	茚虫威	1.34±0.21	5.43(3.83~7.91)	1.12(4)	31.94
R	乙基多杀菌素	1.46±0.22	0.86(0.61~1.19)	2.24(4)	43.00
S	茚虫威	1.33±0.23	0.17 (0.10~0.25)	1.04(4)	-
S	乙基多杀菌素	1.42±0.22	0.02(0.010~0.022)	2.76(4)	-
S♀×R♂	茚虫威	1.42±0.25	1.16(0.62~1.72)	2.81(4)	6.82
S♀×R♂	乙基多杀菌素	1.67±0.25	0.43(0.29~0.58)	0.42(4)	21.50
S♂×R♀	茚虫威	1.59±0.24	1.82(1.20~2.51)	4.02(4)	10.71
S♂×R♀	乙基多杀菌素	1.37±0.22	0.51(0.33~0.72)	2.18(4)	25.50
R♂×R♀	茚虫威	1.31±0.21	4.67(3.25~6.77)	0.94(4)	27.47
R♂×R♀	乙基多杀菌素	1.47±0.22	0.82(0.58~1.13)	1.55(4)	41.00
S♂×S♀	茚虫威	1.38±0.23	0.15(0.09~0.22)	2.84(4)	-
S♂×S♀	乙基多杀菌素	1.81±0.29	0.02(0.011~0.022)	1.39(4)	-

种群	药剂	斜率±标准误	LC₅₀(95%置信区间)/(mg/L)	X²(df)	抗性倍数
R	茚虫威	1.34±0.21	5.43(3.83~7.91)	1.12(4)	31.94
R	乙基多杀菌素	1.46±0.22	0.86(0.61~1.19)	2.24(4)	43.00
S	茚虫威	1.33±0.23	0.17 (0.10~0.25)	1.04(4)	-
S	乙基多杀菌素	1.42±0.22	0.02(0.010~0.022)	2.76(4)	-
S♀×R♂	茚虫威	1.42±0.25	1.16(0.62~1.72)	2.81(4)	6.82
S♀×R♂	乙基多杀菌素	1.67±0.25	0.43(0.29~0.58)	0.42(4)	21.50
S♂×R♀	茚虫威	1.59±0.24	1.82(1.20~2.51)	4.02(4)	10.71
S♂×R♀	乙基多杀菌素	1.37±0.22	0.51(0.33~0.72)	2.18(4)	25.50
R♂×R♀	茚虫威	1.31±0.21	4.67(3.25~6.77)	0.94(4)	27.47
R♂×R♀	乙基多杀菌素	1.47±0.22	0.82(0.58~1.13)	1.55(4)	41.00
S♂×S♀	茚虫威	1.38±0.23	0.15(0.09~0.22)	2.84(4)	-
S♂×S♀	乙基多杀菌素	1.81±0.29	0.02(0.011~0.022)	1.39(4)	-

处理	产卵量/ （粒/雌）	产卵历期/d	卵孵化率/%	化蛹率/%	历期/d			蛹重/(mg/个)	羽化率/%
处理	产卵量/ （粒/雌）	产卵历期/d	卵孵化率/%	化蛹率/%	1龄到4龄幼虫	幼虫→蛹	蛹→成虫	蛹重/(mg/个)	羽化率/%
S♀×R♂	(70±18.08)b	(4.21±0.43)ab	(81±0.08) a	(71±0.14) a	(6.14±0.66)a	8	5	(6.21±1.36) a	(41.86±0.10)a
S♂×R♀	(87±16.62)a	(4.00±0.00)b	(71±0.21)ab	(69±0.18)a	(6.17±0.58)a	8	5	(5.31±0.79)ab	(43.00±0.31)a
R♂×R♀	(72±13.15)b	(4.18±0.40)ab	(73±0.14)ab	(65±0.09)a	(6.73±0.90)a	8	5	(6.11±1.36)a	(42.36±0.07)a
S♂×S♀	(61±18.70)b	(4.44±0.53)a	(64±0.14)b	(60±0.15)a	(5.22±0.44)b	8	5	(4.61±0.50)b	(44.33±0.12)a

处理	产卵量/ （粒/雌）	产卵历期/d	卵孵化率/%	化蛹率/%	历期/d			蛹重/(mg/个)	羽化率/%
处理	产卵量/ （粒/雌）	产卵历期/d	卵孵化率/%	化蛹率/%	1龄到4龄幼虫	幼虫→蛹	蛹→成虫	蛹重/(mg/个)	羽化率/%
S♀×R♂	(70±18.08)b	(4.21±0.43)ab	(81±0.08) a	(71±0.14) a	(6.14±0.66)a	8	5	(6.21±1.36) a	(41.86±0.10)a
S♂×R♀	(87±16.62)a	(4.00±0.00)b	(71±0.21)ab	(69±0.18)a	(6.17±0.58)a	8	5	(5.31±0.79)ab	(43.00±0.31)a
R♂×R♀	(72±13.15)b	(4.18±0.40)ab	(73±0.14)ab	(65±0.09)a	(6.73±0.90)a	8	5	(6.11±1.36)a	(42.36±0.07)a
S♂×S♀	(61±18.70)b	(4.44±0.53)a	(64±0.14)b	(60±0.15)a	(5.22±0.44)b	8	5	(4.61±0.50)b	(44.33±0.12)a