Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (27): 118-125.doi: 10.11924/j.issn.1000-6850.casb2022-0971
Previous Articles Next Articles
QI Kexiang1(), GUAN Xin1, YOU Mengyao1, ZHANG He1, WANG Yuqing1, CHENG Li2(), ZHENG Chunying1()
Received:
2022-11-23
Revised:
2023-03-15
Online:
2023-09-25
Published:
2023-09-22
QI Kexiang, GUAN Xin, YOU Mengyao, ZHANG He, WANG Yuqing, CHENG Li, ZHENG Chunying. The Endophytic Bacterium HS8: Isolation of Secondary Metabolites Based on Flavonoids Biosynthesis[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 118-125.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2022-0971
仪器 | 型号规格 | 生产厂家 |
---|---|---|
电子精密天平 | PL303 | 梅特勒-托利多仪器有限公司 |
超净工作台 | SZX | 南通科学仪器有限公司 |
空气浴振荡器 | HZQ-C | 哈尔滨市东联电子技术开发有限公司 |
旋转蒸发仪 | R-201 | 上海申胜生物技术有限公司 |
超声波清洗器 | KQ-100DE | 昆山市超声仪器有限公司 |
紫外可见波长检测器 | FL2000 | 浙江温岭福立分析仪器有限公司 |
高效液相色谱仪 | FL2000 | 浙江温岭福立分析仪器有限公司 |
400MH固体超导核磁共振波谱仪 | AVANCE Ⅲ | 美国Thermo Fisher公司 |
仪器 | 型号规格 | 生产厂家 |
---|---|---|
电子精密天平 | PL303 | 梅特勒-托利多仪器有限公司 |
超净工作台 | SZX | 南通科学仪器有限公司 |
空气浴振荡器 | HZQ-C | 哈尔滨市东联电子技术开发有限公司 |
旋转蒸发仪 | R-201 | 上海申胜生物技术有限公司 |
超声波清洗器 | KQ-100DE | 昆山市超声仪器有限公司 |
紫外可见波长检测器 | FL2000 | 浙江温岭福立分析仪器有限公司 |
高效液相色谱仪 | FL2000 | 浙江温岭福立分析仪器有限公司 |
400MH固体超导核磁共振波谱仪 | AVANCE Ⅲ | 美国Thermo Fisher公司 |
鉴定指标 | HS结果 | 鉴定指标 | HS8结果 |
---|---|---|---|
菌落形态 | 圆形 | 甲基红试验 | + |
菌落颜色 | 乳白色 | 运动性试验 | + |
革兰氏染色 | - | V.P试验 | + |
细胞形态 | 杆菌 | 菌膜形成 | + |
鞭毛 | + | 最适生长温度 | 37℃ |
淀粉水解反应 | + | 需氧性试验 | 好氧菌 |
明胶液化试验 | + | 最适盐浓度 | 4% NaCl |
接触酶试验 | + | 最适生长pH值 | 7.0 |
油脂水解实验 | + | 对数生长期 | 10~20 h |
G+/G- | + | 硝酸盐还原 | + |
鉴定指标 | HS结果 | 鉴定指标 | HS8结果 |
---|---|---|---|
菌落形态 | 圆形 | 甲基红试验 | + |
菌落颜色 | 乳白色 | 运动性试验 | + |
革兰氏染色 | - | V.P试验 | + |
细胞形态 | 杆菌 | 菌膜形成 | + |
鞭毛 | + | 最适生长温度 | 37℃ |
淀粉水解反应 | + | 需氧性试验 | 好氧菌 |
明胶液化试验 | + | 最适盐浓度 | 4% NaCl |
接触酶试验 | + | 最适生长pH值 | 7.0 |
油脂水解实验 | + | 对数生长期 | 10~20 h |
G+/G- | + | 硝酸盐还原 | + |
[1] |
|
[2] |
doi: 10.1016/j.ejbas.2018.07.002 URL |
[3] |
翟李欣, 徐慧超, 李伟, 等. 东北膜荚黄芪内生细菌的分离及其活性代谢物分析[J]. 中国食品学报, 2019, 19(3):299-305.
|
[4] |
|
[5] |
国家药典委员会. 中华人民共和国药典2020年版(一部)[S]. 北京:中国医药科技出版社, 2020:302-303.
|
[6] |
|
[7] |
|
[8] |
|
[9] |
|
[10] |
|
[11] |
|
[12] |
曾燕, 郭兰萍, 王继永, 等. 植物黄酮类化合物的化学生态学功能及中药材定向栽培的构想[J]. 中国现代中药, 2015, 17(8):776-790.
|
[13] |
|
[14] |
doi: 10.1021/jf60120a015 URL |
[15] |
|
[16] |
|
[17] |
|
[18] |
|
[19] |
|
[20] |
|
[21] |
|
[22] |
|
[23] |
|
[24] |
邹小周, 曹张军, 陈琳, 等. 四株细菌纤维素生产菌株16SrDNA序列分析及生产性能比较研究[J]. 纤维素科学与技术, 2018, 26(4):1-8.
|
[25] |
|
[26] |
|
[27] |
徐晓杰. LED光对黄芪毛状根生长及芒柄花素和毛蕊异黄酮生物合成的影响[D]. 哈尔滨: 东北林业大学, 2022, 64(6):12-23.
|
[28] |
白月, 王红芳, 黄怀鹏. 芒柄花素药理作用的研究进展[J]. 现代药物与临床, 2022, 37(2):425-432.
|
[29] |
doi: 10.1016/j.phymed.2022.154277 URL |
[1] | CHEN Lulu, WANG Huili. Drying Method and Component Analysis of Apios americana [J]. Chinese Agricultural Science Bulletin, 2023, 39(4): 154-159. |
[2] | GUAN Xin, QI Kexiang, LI Wanru, ZHANG He, JIANG Shuo, WU Tong, ZHENG Chunying. CBD and CBDA Contents in Fermented Cannabis sativa L.: Study of Dynamic Changes [J]. Chinese Agricultural Science Bulletin, 2023, 39(21): 144-150. |
[3] | LI Rumei, LIU Tongjin, LIANG Hui, FENG Yizhi, LIANG Lin, LI Ruijuan, YU Jianlei. Residue Behavior of Thiamethoxam in Chinese Flowering Cabbage Analyzed by Performance Liquid Chromatography-tandem Mass Spectrometry [J]. Chinese Agricultural Science Bulletin, 2023, 39(20): 133-137. |
[4] | WU Guifen, YAN Huandan. Comparative Analysis of Total Flavonoids Content in Wild Macrofungi [J]. Chinese Agricultural Science Bulletin, 2023, 39(13): 125-129. |
[5] | LI Hanqiu, DU Jingyan, MANZILA·Tudaji, SONG Xinyu, TIAN Yelin, WANG Jianfang. Three Medicinal Ingredients of Atractylodes chinensis (DC.) Koidz: Extraction and Determination Based on HPLC [J]. Chinese Agricultural Science Bulletin, 2023, 39(11): 43-49. |
[6] | ZHENG Lingling, JI Ruifeng, HAN Hongliang, LUO Yilu, LUO Jiayi, GUAN Yueqin, YAO Qisheng, ZHENG Weibing, CHEN Meilan, ZHOU Xiuteng. Determination of Five Kinds of Flavonoids in the Medicinal Herbs of Thesium chinensis [J]. Chinese Agricultural Science Bulletin, 2022, 38(5): 30-36. |
[7] | FANG Jiamin, ZENG Weimin, ZHANG Yanlong. The Contents and Anti-tumor Activity of Main Chemical Components of Scutellaria baicalensis from Different Origins: A Comparative Study [J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 120-126. |
[8] | YOU Mengyao, WAN Lu, YAN Jiajia, ZHANG He, ZHENG Chunying. Research Progress on Endophytes from Glycyrrhiza uralensis [J]. Chinese Agricultural Science Bulletin, 2022, 38(26): 20-26. |
[9] | DONG Shiliang, ZHAN Siwen, HE Jiewang, QIU Tong, ZHANG Xueqiong, LIU Fengfeng. Quality Classification of Flue-cured Tobacco Based on HPLC Fingerprint Technique [J]. Chinese Agricultural Science Bulletin, 2022, 38(25): 151-157. |
[10] | WANG Xiaonan, CHANG Hong, WANG Siwei, GU Yanping, SUN Haibin, LIU Yanping. Residue Analysis and Safety Evaluation of Etoxazole in Loquat by HPLC-MS/MS [J]. Chinese Agricultural Science Bulletin, 2022, 38(22): 121-125. |
[11] | ZU Weixi, ZHAO Xiaoxia, GAO Fen. Quality Promotion and Disease Control Effects of Bacillus Strain R57 on Astragalus membranaceus var. mongholicus and Its Identification [J]. Chinese Agricultural Science Bulletin, 2022, 38(21): 129-135. |
[12] | QIN Zhihua, CAI Qingxia, WANG Jianlin, WANG Shubai, TAN Zichao, ZHANG Linlin, GUO Pei, SHAN Hu. Optimization of the Extraction Process of Total Flavonoids from Broussonetia papyrifera Leaves by Ethanol Heating Reflux Method [J]. Chinese Agricultural Science Bulletin, 2022, 38(17): 110-114. |
[13] | Huang Jing, Chen Chan, Huang Xiaomei. Flavonoids from Fujian Bergamot: Ultrasonicly Extracting Technology and Its Antioxidation Properties [J]. Chinese Agricultural Science Bulletin, 2021, 37(7): 126-131. |
[14] | Qian Chunrong, Li Liang, Jiang Yubo, Yu Yang, Hao Yubo, Gong Xiujie, Ge Xuanliang, Lv Guoyi, Wang Weiming, Lai Yongcai. Effects of Harvesting Period of Corn Silk on Its Functional Components, Collectable Silk Yield and Maize Yield [J]. Chinese Agricultural Science Bulletin, 2021, 37(7): 14-18. |
[15] | Zhang Zhedong, Liang Jing, Li Zeyu, Gao Siyu, Qiu Tianyi, Shan Tijiang, Xu Lijian. Litter Fungi in the Boreal Forests and Their Antibacterial Compounds in the Greater Khingan Mountains [J]. Chinese Agricultural Science Bulletin, 2021, 37(6): 104-110. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||