Potato Breeding Material in Kazakhstan: Viruses Identification and Dry Rot Resistance

doi:10.11924/j.issn.1000-6850.casb2022-0969

Abstract

Abstract:

This paper aims to reveal the resistance of potato breeding materials to potato dry rot disease and the infection of potato virus disease. Enzyme linked immunosorbent assay (ELISA) and Fusarium oxysporum infection were used to identify the virus infection status and dry rot resistance of 38 potato breeding materials. The results showed that the occurrence of potato virus disease in Akmola was complex, and there were multiple virus infections. Among 38 materials, 18 strains and 1 variety material were not infected with potato virus. The resistance identification of Fusarium oxysporum in 18 potato breeding materials, including 1 resistant material, 1 medium resistant material, and the rest not possessing disease resistance. The occurrence of potato virus diseases in the Aklamo region of Kazakhstan was complex, and it was necessary to pay attention to the detoxification of seed potatoes and the prevention and control of virus transmission. Dry rot resistant materials could be used as breeding parent materials.

Key words: potato, viral diseases, dry rot disease, Fusarium oxysporum, enzyme linked immunosorbent assay, resistance

WANG Zhenzhen, AZIMAKHAN Moldir, SERIKOVA Zhuldyz, KHASSANOV Vadim, TULEEVA Asiya, ZHANG Zhikai, LV Dianqiu, HU Baigeng. Potato Breeding Material in Kazakhstan: Viruses Identification and Dry Rot Resistance[J]. Chinese Agricultural Science Bulletin, 2023, 39(24): 127-134.

Figures/Tables 6

References 26

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原编号	样本编号	PVX	Ao/Ok	检测结果	PVY	Ao/Ok	检测结果	PVS	Ao/Ok	检测结果	PVM	Ao/Ok	检测结果	选择结果
17250-10	1	0.312	3.7	+	0.221	3.0	+	0.195	2.9	-	0.258	3.9	+	淘汰
17 228-7	2	0.136	1.2	-	0.135	1.5	-	0.136	1.7	-	0.165	2.2	-	选择
17223-10	3	0.184	1.9	-	0.195	2.5	-	0.148	2.0	-	0.181	2.5	-	选择
17 250-1	4	0.070	0.3	-	0.084	0.6	-	0.080	0.6	-	0.184	2.5	-	选择
17250-11	5	0.085	0.5	-	0.055	0.1	-	0.067	0.3	-	0.293	4.6	+	淘汰
Z 879	6	0.134	1.2	-	0.057	0.1	-	0.133	1.7	-	0.987	17.7	+	淘汰
Z765-1	7	0.152	1.5	-	0.147	1.7	-	0.145	1.9	-	0.142	1.7	-	选择
17241-4	8	0.214	2.3	-	0.176	2.2	-	0.160	2.2	-	0.498	8.5	+	淘汰
17212-19	9	0.187	2.0	-	0.144	1.6	-	0.124	1.5	-	0.505	8.6	+	淘汰
Z 897-3	10	0.123	1.0	-	0.116	1.2	-	0.082	0.6	-	0.102	1.0	-	选择
17203-8	11	0.224	2.5	-	0.147	1.7	-	0.180	2.6	-	0.331	5.3	+	淘汰
17 249	12	0.152	1.5	-	0.047	0.1	-	0.148	2.0	-	0.187	2.6	-	选择
17222-15	13	0.107	0.8	-	0.095	0.8	-	0.079	0.6	-	0.098	0.9	-	选择
17223-6	14	0.283	3.3	+	0.254	3.6	+	0.194	2.9	-	0.290	4.5	+	淘汰
17212-45	15	0.202	2.2	-	0.222	3.0	+	0.157	2.1	-	0.207	3.0	+	淘汰
17211-20	16	0.160	1.6	-	0.174	2.2	-	0.127	1.5	-	0.163	2.1	-	选择
17215-11	17	0.120	1.0	-	0.082	0.6	-	0.079	0.6	-	0.318	5.1	+	淘汰
17236-7	18	0.167	1.7	-	0.040	0.2	-	0.151	2.0	-	0.230	3.4	+	淘汰
Z 440 3	19	0.101	0.7	-	0.096	0.8	-	0.066	0.3	-	0.092	0.8	-	选择
17243-4	20	0.150	1.4	-	0.137	1.5	-	0.122	1.4	-	0.312	4.9	+	淘汰
17 212-18	21	0.147	1.4	-	0.125	1.3	-	0.086	0.7	-	0.268	4.1	+	淘汰
17214-9	22	0.170	1.7	-	0.188	2.4	-	0.153	2.1	-	0.150	1.9	-	选择
17235-3	23	0.180	1.9	-	0.150	1.8	-	0.132	1.6	-	0.198	2.8	-	选择
Z 861-1	24	0.125	1.1	-	0.028	0.4	-	0.096	0.9	-	0.196	2.8	-	选择
17223-2	25	0.541	7.0	+	0.430	6.7	+	0.377	6.5	+	0.390	6.4	+	淘汰
17242-8	26	0.322	3.9	+	0.262	3.7	+	0.244	3.9	+	0.274	4.2	+	淘汰
17201-8	27	0.310	3.7	+	0.161	1.9	-	0.110	1.2	-	0.122	1.4	-	淘汰
17 99	28	0.194	2.1	-	0.194	2.5	-	0.136	1.7	-	0.145	1.8	-	选择
17201-11	29	0.147	1.4	-	0.149	1.7	-	0.139	1.8	-	0.172	2.3	-	选择
17225-11	30	0.169	1.7	-	0.034	0.3	-	0.113	1.3	-	0.190	2.6	-	选择
17205-6	31	0.117	1.0	-	0.123	1.3	-	0.102	1.0	-	0.128	1.5	-	选择
17249-2	32	0.113	0.9	-	0.149	1.7	-	0.121	1.4	-	0.188	2.6	-	选择
17214-2	33	0.157	1.5	-	0.241	3.4	+	0.208	3.2	+	0.314	5.0	+	淘汰
17225-12	34	0.207	2.2	-	0.180	2.3	-	0.152	2.0	-	0.209	3.0	+	淘汰
17243-5	35	0.278	3.3	+	0.257	3.6	+	0.220	3.4	+	0.278	4.3	+	淘汰
17 204-2	36	0.128	1.1	-	0.044	0.1	-	0.098	1.0	-	0.181	2.5	-	选择
17 216-3	37	0.143	1.3	-	0.310	4.6	+	0.293	4.9	+	0.358	5.8	+	淘汰
Xisen 6	38	0.139	1.1	-	0.101	0.9	-	0.124	1.2	-	0.120	1.1	-	选择
PC (+)		0.800	6.7	+	0.600	5.6	+	0.650	6.5	+	0.720	6.9903	+	淘汰
NC (-)		0.120	-	-	0.107	-	-	0.100	-	-	0.103	-	-	淘汰

原编号	样本编号	PVX	Ao/Ok	检测结果	PVY	Ao/Ok	检测结果	PVS	Ao/Ok	检测结果	PVM	Ao/Ok	检测结果	选择结果
17250-10	1	0.312	3.7	+	0.221	3.0	+	0.195	2.9	-	0.258	3.9	+	淘汰
17 228-7	2	0.136	1.2	-	0.135	1.5	-	0.136	1.7	-	0.165	2.2	-	选择
17223-10	3	0.184	1.9	-	0.195	2.5	-	0.148	2.0	-	0.181	2.5	-	选择
17 250-1	4	0.070	0.3	-	0.084	0.6	-	0.080	0.6	-	0.184	2.5	-	选择
17250-11	5	0.085	0.5	-	0.055	0.1	-	0.067	0.3	-	0.293	4.6	+	淘汰
Z 879	6	0.134	1.2	-	0.057	0.1	-	0.133	1.7	-	0.987	17.7	+	淘汰
Z765-1	7	0.152	1.5	-	0.147	1.7	-	0.145	1.9	-	0.142	1.7	-	选择
17241-4	8	0.214	2.3	-	0.176	2.2	-	0.160	2.2	-	0.498	8.5	+	淘汰
17212-19	9	0.187	2.0	-	0.144	1.6	-	0.124	1.5	-	0.505	8.6	+	淘汰
Z 897-3	10	0.123	1.0	-	0.116	1.2	-	0.082	0.6	-	0.102	1.0	-	选择
17203-8	11	0.224	2.5	-	0.147	1.7	-	0.180	2.6	-	0.331	5.3	+	淘汰
17 249	12	0.152	1.5	-	0.047	0.1	-	0.148	2.0	-	0.187	2.6	-	选择
17222-15	13	0.107	0.8	-	0.095	0.8	-	0.079	0.6	-	0.098	0.9	-	选择
17223-6	14	0.283	3.3	+	0.254	3.6	+	0.194	2.9	-	0.290	4.5	+	淘汰
17212-45	15	0.202	2.2	-	0.222	3.0	+	0.157	2.1	-	0.207	3.0	+	淘汰
17211-20	16	0.160	1.6	-	0.174	2.2	-	0.127	1.5	-	0.163	2.1	-	选择
17215-11	17	0.120	1.0	-	0.082	0.6	-	0.079	0.6	-	0.318	5.1	+	淘汰
17236-7	18	0.167	1.7	-	0.040	0.2	-	0.151	2.0	-	0.230	3.4	+	淘汰
Z 440 3	19	0.101	0.7	-	0.096	0.8	-	0.066	0.3	-	0.092	0.8	-	选择
17243-4	20	0.150	1.4	-	0.137	1.5	-	0.122	1.4	-	0.312	4.9	+	淘汰
17 212-18	21	0.147	1.4	-	0.125	1.3	-	0.086	0.7	-	0.268	4.1	+	淘汰
17214-9	22	0.170	1.7	-	0.188	2.4	-	0.153	2.1	-	0.150	1.9	-	选择
17235-3	23	0.180	1.9	-	0.150	1.8	-	0.132	1.6	-	0.198	2.8	-	选择
Z 861-1	24	0.125	1.1	-	0.028	0.4	-	0.096	0.9	-	0.196	2.8	-	选择
17223-2	25	0.541	7.0	+	0.430	6.7	+	0.377	6.5	+	0.390	6.4	+	淘汰
17242-8	26	0.322	3.9	+	0.262	3.7	+	0.244	3.9	+	0.274	4.2	+	淘汰
17201-8	27	0.310	3.7	+	0.161	1.9	-	0.110	1.2	-	0.122	1.4	-	淘汰
17 99	28	0.194	2.1	-	0.194	2.5	-	0.136	1.7	-	0.145	1.8	-	选择
17201-11	29	0.147	1.4	-	0.149	1.7	-	0.139	1.8	-	0.172	2.3	-	选择
17225-11	30	0.169	1.7	-	0.034	0.3	-	0.113	1.3	-	0.190	2.6	-	选择
17205-6	31	0.117	1.0	-	0.123	1.3	-	0.102	1.0	-	0.128	1.5	-	选择
17249-2	32	0.113	0.9	-	0.149	1.7	-	0.121	1.4	-	0.188	2.6	-	选择
17214-2	33	0.157	1.5	-	0.241	3.4	+	0.208	3.2	+	0.314	5.0	+	淘汰
17225-12	34	0.207	2.2	-	0.180	2.3	-	0.152	2.0	-	0.209	3.0	+	淘汰
17243-5	35	0.278	3.3	+	0.257	3.6	+	0.220	3.4	+	0.278	4.3	+	淘汰
17 204-2	36	0.128	1.1	-	0.044	0.1	-	0.098	1.0	-	0.181	2.5	-	选择
17 216-3	37	0.143	1.3	-	0.310	4.6	+	0.293	4.9	+	0.358	5.8	+	淘汰
Xisen 6	38	0.139	1.1	-	0.101	0.9	-	0.124	1.2	-	0.120	1.1	-	选择
PC (+)		0.800	6.7	+	0.600	5.6	+	0.650	6.5	+	0.720	6.9903	+	淘汰
NC (-)		0.120	-	-	0.107	-	-	0.100	-	-	0.103	-	-	淘汰

原编号	稳定性得分	感染率/%	稳定性程度
17214-9	3	50	低
17211-20	3	45	低
17222-15	3	55	低
17235-3	3	60	低
17228-7	3	56	低
Z 861-1	5	38	中等
Z 897-3	3	45	低
1799	3	47	低
Z 765-1	3	45	低
17 250-1	3	88	极低
17 204-2	3	70	极低
17 223-10	3	60	低
17 249	1	75	极低
17 201-11	3	50	低
Z 440-3	7	20	较高
17 225-11	3	58	低
17205-6	3	60	低
17249-2	1	75	极低
Xisen 6 (control)	8	10	较高

原编号	稳定性得分	感染率/%	稳定性程度
17214-9	3	50	低
17211-20	3	45	低
17222-15	3	55	低
17235-3	3	60	低
17228-7	3	56	低
Z 861-1	5	38	中等
Z 897-3	3	45	低
1799	3	47	低
Z 765-1	3	45	低
17 250-1	3	88	极低
17 204-2	3	70	极低
17 223-10	3	60	低
17 249	1	75	极低
17 201-11	3	50	低
Z 440-3	7	20	较高
17 225-11	3	58	低
17205-6	3	60	低
17249-2	1	75	极低
Xisen 6 (control)	8	10	较高