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Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (27): 126-132.doi: 10.11924/j.issn.1000-6850.casb2022-0815

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Effects of Hybridization on Growth and Development and Detoxification Metabolic Enzyme Activity of Plutella xylostella Progenies

YIN Fei(), GE Tiancheng, XIAO Yong, PENG Zhengke, LI Zhenyu()   

  1. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640
  • Received:2022-09-26 Revised:2022-12-29 Online:2023-09-25 Published:2023-09-22

Abstract:

The research aims to clarify the effect of hybridization between susceptible and resistant diamondback moth (DBM) on the growth, development and resistance of its progeny, and to explore new methods for the resistance management of DBM. In this study, the susceptible population hybridized with field resistant population of Plutella xylostella. The growth and development, sensitivity to pesticides and detoxification metabolic enzyme activity in F1 generation of diamondback moth were analyzed. The results showed that the hybridization of susceptible and resistant diamondback moth had no significant effect on the fecundity, developmental duration, pupation rate and emergence rate of Plutella xylostella. The sensitivity of DBM orthogonal and reverse cross populations F1 to indoxacarb and spinetoram was improved. The resistant ratio of DBM orthogonal F1 to indoxacarb and spinetoram decreased more obviously. Compared with the resistant population, the resistant ratio decreased from 27.47 or 41.00 times to 6.82 or 21.50 times, respectively. The activities of glutathione-s transferase (GSTs) in the hybrid offspring of diamondback moth decreased significantly. The enzyme activities of DBM orthogonal and reverse cross populations decreased by 67.29% and 53.80%, respectively. After hybridization between sensitive and resistant diamondback moth, the sensitivity of diamondback moth to pesticides may be affected by regulating the activity of metabolic enzymes. The results can provide a new idea for the management of diamondback moth resistance.

Key words: Plutella xylostella, hybridization, pesticides, resistance, metabolic enzyme