Effects of Temperature on Growth, Physiology and Photosynthetic Characteristics of Brassica pekinensis Seedlings

doi:10.11924/j.issn.1000-6850.casb2024-0705

Abstract

Abstract:

The growth, physiological and photosynthetic characteristics of Brassica pekinensis seedlings under different temperature conditions were analyzed to determine the optimal temperature range for their growth. Taking B. pekinensis ‘Cold-resistant Golden Empress’ as test material, a completely randomized block design was used to set up eight temperature treatments of 0, 5, 10, 15, 20, 25 (control), 30 and 35℃. The plant height, root diameter, chlorophyll content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, malondialdehyde (MDA) content, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), intercellular CO₂ concentration (Ci) were measured. The results showed that the seedlings began to die at 0℃. The plant height and root collar diameter increased slowly at 5-15℃ and 30-35℃. At 20℃, the plant height and root collar diameter were the largest, and the growth was good. The chlorophyll content, SOD activity and POD activity in the plant showed a trend of increasing first and then decreasing, and the MDA activity showed a trend of decreasing first and then increasing. With the change of time, Tr, Gs, Pn and Ci of B. pekinensis showed an upward trend. The Pn reached the maximum at 20℃, and the Gs, Tr and Ci of B. pekinensis seedlings were the largest at 35℃. Temperature lower than 20℃ or higher than 25℃ would affect the growth of B. pekinensis seedlings, resulting in a decrease in leaf enzyme activity and photosynthetic capacity. The optimum temperature range for the growth of B. pekinensis seedlings was 20-25℃, and the plant morphogenesis, physiological metabolism and photosynthetic performance were the best in this range.

Key words: Brassica pekinensis, seedlings, temperature, physiological characteristics, photosynthetic parameters, growth index, duress, resistance

E Lifeng, YAO Furong, CHEN Xiubin, QUAN Jianhua, HUA Jun, YIN Lijuan, ZHAO Wenqin. Effects of Temperature on Growth, Physiology and Photosynthetic Characteristics of Brassica pekinensis Seedlings[J]. Chinese Agricultural Science Bulletin, 2025, 41(16): 28-34.

Figures/Tables 7

References 25

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时间/d	温度/℃	净光合速率(Pn)/	气孔导度(Gs)/	蒸腾速率(Tr)/	胞间CO₂浓度(Ci)/
时间/d	温度/℃	[μmolCO₂/(m²·s)]	[molH₂O/(m²·s)]	[mmolH₂O/(m²·s)]	[μmolCO₂/(m²·s)]
3	0	5.77±0.42ab	150.67±6.66ab	4.95±0.44ab	374.33±221.87a
	5	6.70±1.57a	129.33±14.01ab	4.76±0.38ab	306.00±21.00a
	10	4.03±2.40ab	112.33±18.90b	4.24±0.48b	335.33±28.02a
	15	4.10±1.21ab	109.33±15.50b	4.20±0.42b	337.00±10.00a
	20	3.97±0.55ab	112.67±9.71b	4.50±0.28b	347.00±39.95a
	25	4.70±3.26ab	113.00±27.51b	4.43±0.75b	324.67±33.02a
	30	2.67±1.51b	138.67±33.201ab	5.09±0.71ab	360.67±17.56a
	35	6.27±3.07ab	211.00±132.50a	6.34±2.30a	338.67±25.32a
6	0	6.27±0.90b	79.67±17.47ab	4.10±0.98ab	120.33±51.16c
	5	4.73±0.21c	77.67±4.04ab	3.68±0.16ab	256.67±134.11b
	10	4.60±0.87c	83.33±6.51ab	4.04±0.37ab	303.33±13.05ab
	15	4.13±0.32c	95.00±13.00a	2.92±0.09b	358.67±64.30ab
	20	7.47±0.49a	85.33±1.53ab	4.48±0.48a	340.67±39.72ab
	25	4.73±0.40c	82.33±4.16ab	3.56±1.18ab	378.33±18.18a
	30	4.00±0.44c	70.33±17.21b	2.93±0.25b	291.67±20.98ab
	35	4.13±1.18c	68.33±16.50b	2.94±0.67b	313.33±31.66ab
9	0	6.27±0.91b	79.67±17.47ab	4.1±0.983abc	120.33±51.16c
	5	1.70±0.17e	85.67±4.04ab	5.1±0.110abc	357.67±7.02ab
	10	3.40±1.25d	95.00±13.00ab	6.1±0.366abc	303.33±13.05b
	15	4.73±0.60bcd	99.00±16.09a	7.1±0.476a	367.00±38.94ab
	20	8.37±1.16a	91.33±5.51ab	8.1±0.424ab	347.33±19.14b
	25	5.10±0.20bc	91.33±5.03ab	9.1±0.681abc	427.00±59.93a
	30	5.13±0.60bc	75.33±12.22ab	10.1±0.695bc	357.67±36.12ab
	35	3.93±1.17cd	71.67±20.53b	11.1±0.746c	320.33±42.44b

时间/d	温度/℃	净光合速率(Pn)/	气孔导度(Gs)/	蒸腾速率(Tr)/	胞间CO₂浓度(Ci)/
时间/d	温度/℃	[μmolCO₂/(m²·s)]	[molH₂O/(m²·s)]	[mmolH₂O/(m²·s)]	[μmolCO₂/(m²·s)]
3	0	5.77±0.42ab	150.67±6.66ab	4.95±0.44ab	374.33±221.87a
	5	6.70±1.57a	129.33±14.01ab	4.76±0.38ab	306.00±21.00a
	10	4.03±2.40ab	112.33±18.90b	4.24±0.48b	335.33±28.02a
	15	4.10±1.21ab	109.33±15.50b	4.20±0.42b	337.00±10.00a
	20	3.97±0.55ab	112.67±9.71b	4.50±0.28b	347.00±39.95a
	25	4.70±3.26ab	113.00±27.51b	4.43±0.75b	324.67±33.02a
	30	2.67±1.51b	138.67±33.201ab	5.09±0.71ab	360.67±17.56a
	35	6.27±3.07ab	211.00±132.50a	6.34±2.30a	338.67±25.32a
6	0	6.27±0.90b	79.67±17.47ab	4.10±0.98ab	120.33±51.16c
	5	4.73±0.21c	77.67±4.04ab	3.68±0.16ab	256.67±134.11b
	10	4.60±0.87c	83.33±6.51ab	4.04±0.37ab	303.33±13.05ab
	15	4.13±0.32c	95.00±13.00a	2.92±0.09b	358.67±64.30ab
	20	7.47±0.49a	85.33±1.53ab	4.48±0.48a	340.67±39.72ab
	25	4.73±0.40c	82.33±4.16ab	3.56±1.18ab	378.33±18.18a
	30	4.00±0.44c	70.33±17.21b	2.93±0.25b	291.67±20.98ab
	35	4.13±1.18c	68.33±16.50b	2.94±0.67b	313.33±31.66ab
9	0	6.27±0.91b	79.67±17.47ab	4.1±0.983abc	120.33±51.16c
	5	1.70±0.17e	85.67±4.04ab	5.1±0.110abc	357.67±7.02ab
	10	3.40±1.25d	95.00±13.00ab	6.1±0.366abc	303.33±13.05b
	15	4.73±0.60bcd	99.00±16.09a	7.1±0.476a	367.00±38.94ab
	20	8.37±1.16a	91.33±5.51ab	8.1±0.424ab	347.33±19.14b
	25	5.10±0.20bc	91.33±5.03ab	9.1±0.681abc	427.00±59.93a
	30	5.13±0.60bc	75.33±12.22ab	10.1±0.695bc	357.67±36.12ab
	35	3.93±1.17cd	71.67±20.53b	11.1±0.746c	320.33±42.44b