Study on Antioxidant Activity of Extracts from 16 Plants in Inner Mongolia Grassland

doi:10.11924/j.issn.1000-6850.casb2024-0734

Abstract

Abstract:

To investigate the antioxidant activity of plant extracts from Inner Mongolia grassland, 16 grassland plant species collected from Dalate Banner and Ulgai grassland were used as experimental materials. The contents of total phenolic, total flavonoid, total polysaccharide, and total saponin in the extracts were determined using the Folin-Ciocalteu method, aluminum chloride colorimetric method, concentrated sulfuric acid-phenol colorimetric method, and vanillin-perchloric acid colorimetric method, respectively. Antioxidant activity was evaluated using DPPH and ABTS free radical scavenging rates along with the total antioxidant capacity as indicators. The results showed that significant differences were in the content of these component and antioxidant activity among the 16 plant extracts. Sanguisorba officinalis and Koenigia divaricata exhibited the highest total phenolic content, reaching 33.22% and 29.34%, respectively. Koenigia divaricata also had the highest total flavonoid content, at 17.72%. Moreover, the extracts of Sanguisorba officinalis and Koenigia divaricata exhibited the strongest DPPH and ABTS radical scavenging abilities and the highest total antioxidant capacity. This study clarifies the high antioxidant activity plant resources in Inner Mongolia grassland, and provides scientific theoretical support for the antioxidant functions, pharmaceutical and functional product development of grassland plants, and the sustainable utilization of grassland plant resources.

Key words: grassland plants, plant extracts, total phenolic, total flavonoid, total polysaccharide, total saponin, antioxidant activity

ZHAO Zhihui, DAI Jiyun, LIU Yuning, FU Hui, YING Ying, HUANG Yiying, JIANG Xiaowei, LIU Jiangying, WEI Yuan, ZHANG Yandong, WANG Lixia, YU Haidong, WAN Yumeng, QIU Pengcheng, SHI Fu, LIU Tinglu, SUN Fengwu, WU Hongxin. Study on Antioxidant Activity of Extracts from 16 Plants in Inner Mongolia Grassland[J]. Chinese Agricultural Science Bulletin, 2025, 41(32): 116-123.

Figures/Tables 6

References 36

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编号	采集地	科名	名称	拉丁名
1	达拉特旗	豆科^[15]	甘草	Glycyrrhiza uralensis Fisch.
2	达拉特旗	豆科^[16]	苦豆子	Sophora alopecuroides L.
3	达拉特旗	石蒜科^[17]	蒙古韭	Allium mongolicum Regel
4	达拉特旗	柏科^[18]	沙地柏	Juniperus sabina L.
5	乌拉盖草原	蔷薇科^[19]	地榆	Sanguisorba officinalis L.
6	乌拉盖草原	豆科^[20]	紫花苜蓿	Medicago sativa L.
7	乌拉盖草原	菊科^[21]	长裂苦苣菜	Sonchus brachyotus DC.
8	乌拉盖草原	菊科^[22]	蒲公英	Taraxacum mongolicum Hand.-Mazz.
9	乌拉盖草原	蔷薇科^[23]	星毛委陵菜	Potentilla acaulis L.
10	乌拉盖草原	石竹科^[24]	银柴胡	Stellaria dichotoma var. lanceolata Bunge
11	乌拉盖草原	瑞香科^[25]	狼毒	Stellera chamaejasme L.
12	乌拉盖草原	毛茛科^[26]	唐松草	Thalictrum aquilegiifolium var. sibiricum Regel & Tiling
13	乌拉盖草原	豆科^[27]	多叶棘豆	Oxytropis myriophylla (Pall.) DC.
14	乌拉盖草原	豆科^[28]	斜茎黄芪	Astragalus laxmannii Jacq.
15	乌拉盖草原	蓼科^[29]	叉分蓼	Koenigia divaricata (L.) T. M. Schust. & Reveal
16	乌拉盖草原	景天科^[30]	瓦松	Orostachys fimbriata (Turcz.) A. Berger

编号	采集地	科名	名称	拉丁名
1	达拉特旗	豆科^[15]	甘草	Glycyrrhiza uralensis Fisch.
2	达拉特旗	豆科^[16]	苦豆子	Sophora alopecuroides L.
3	达拉特旗	石蒜科^[17]	蒙古韭	Allium mongolicum Regel
4	达拉特旗	柏科^[18]	沙地柏	Juniperus sabina L.
5	乌拉盖草原	蔷薇科^[19]	地榆	Sanguisorba officinalis L.
6	乌拉盖草原	豆科^[20]	紫花苜蓿	Medicago sativa L.
7	乌拉盖草原	菊科^[21]	长裂苦苣菜	Sonchus brachyotus DC.
8	乌拉盖草原	菊科^[22]	蒲公英	Taraxacum mongolicum Hand.-Mazz.
9	乌拉盖草原	蔷薇科^[23]	星毛委陵菜	Potentilla acaulis L.
10	乌拉盖草原	石竹科^[24]	银柴胡	Stellaria dichotoma var. lanceolata Bunge
11	乌拉盖草原	瑞香科^[25]	狼毒	Stellera chamaejasme L.
12	乌拉盖草原	毛茛科^[26]	唐松草	Thalictrum aquilegiifolium var. sibiricum Regel & Tiling
13	乌拉盖草原	豆科^[27]	多叶棘豆	Oxytropis myriophylla (Pall.) DC.
14	乌拉盖草原	豆科^[28]	斜茎黄芪	Astragalus laxmannii Jacq.
15	乌拉盖草原	蓼科^[29]	叉分蓼	Koenigia divaricata (L.) T. M. Schust. & Reveal
16	乌拉盖草原	景天科^[30]	瓦松	Orostachys fimbriata (Turcz.) A. Berger

植物种类	化学组成%
植物种类	总多酚	总黄酮	总多糖	总皂苷
甘草	11.33±0.14^de	7.16±0.21^c	0.388±0.020^de	0.087±0.004^fg
苦豆子	4.59±0.26^f	0.99±0.01^k	0.309±0.011^f	0.062±0.002^ij
蒙古韭	5.13±0.18^f	2.67±0.11^j	0.445±0.021^bcd	0.121±0.004^bc
沙地柏	10.16±0.59^e	3.67±0.08^hij	0.520±0.030^ab	0.099±0.007^def
地榆	33.22±1.60^a	5.54±0.38^def	0.457±0.007^bcd	0.129±0.008^b
紫花苜蓿	5.49±0.09^f	4.56±0.35^fgh	0.276±0.030^f	0.096±0.004^ef
长裂苦苣菜	5.21±0.32^f	3.47±0.25^ij	0.268±0.011^f	0.097±0.006^def
蒲公英	4.39±0.33^f	4.42±0.02^ghi	0.281±0.013^f	0.051±0.001^ij
星毛委陵菜	12.73±0.69^d	5.86±0.24^de	0.476±0.041^bc	0.169±0.010^a
银柴胡	12.96±0.40^d	9.35±0.23^b	0.335±0.017^ef	0.135±0.004^b
狼毒	22.54±1.37^c	4.74±0.23^fgh	0.434±0.025^cd	0.070±0.003^hi
唐松草	12.65±0.23^d	7.57±0.55^c	0.495±0.040^abc	0.131±0.006^b
多叶棘豆	9.30±0.17^e	6.09±0.37^d	0.285±0.019^f	0.112±0.003^cde
斜茎黄芪	6.10±0.41^f	4.63±0.04^fgh	0.322±0.024^ef	0.078±0.002^gh
叉分蓼	29.34±1.21^b	17.72±0.87^a	0.308±0.028^f	0.070±0.004^hi
瓦松	6.42±0.27^f	4.89±0.32^efg	0.562±0.026^a	0.113±0.005^cd

植物种类	化学组成%
植物种类	总多酚	总黄酮	总多糖	总皂苷
甘草	11.33±0.14^de	7.16±0.21^c	0.388±0.020^de	0.087±0.004^fg
苦豆子	4.59±0.26^f	0.99±0.01^k	0.309±0.011^f	0.062±0.002^ij
蒙古韭	5.13±0.18^f	2.67±0.11^j	0.445±0.021^bcd	0.121±0.004^bc
沙地柏	10.16±0.59^e	3.67±0.08^hij	0.520±0.030^ab	0.099±0.007^def
地榆	33.22±1.60^a	5.54±0.38^def	0.457±0.007^bcd	0.129±0.008^b
紫花苜蓿	5.49±0.09^f	4.56±0.35^fgh	0.276±0.030^f	0.096±0.004^ef
长裂苦苣菜	5.21±0.32^f	3.47±0.25^ij	0.268±0.011^f	0.097±0.006^def
蒲公英	4.39±0.33^f	4.42±0.02^ghi	0.281±0.013^f	0.051±0.001^ij
星毛委陵菜	12.73±0.69^d	5.86±0.24^de	0.476±0.041^bc	0.169±0.010^a
银柴胡	12.96±0.40^d	9.35±0.23^b	0.335±0.017^ef	0.135±0.004^b
狼毒	22.54±1.37^c	4.74±0.23^fgh	0.434±0.025^cd	0.070±0.003^hi
唐松草	12.65±0.23^d	7.57±0.55^c	0.495±0.040^abc	0.131±0.006^b
多叶棘豆	9.30±0.17^e	6.09±0.37^d	0.285±0.019^f	0.112±0.003^cde
斜茎黄芪	6.10±0.41^f	4.63±0.04^fgh	0.322±0.024^ef	0.078±0.002^gh
叉分蓼	29.34±1.21^b	17.72±0.87^a	0.308±0.028^f	0.070±0.004^hi
瓦松	6.42±0.27^f	4.89±0.32^efg	0.562±0.026^a	0.113±0.005^cd