Wheat Grain Deoxynivalenol (DON) Accumulation Dynamics and the Correlation Analysis of Its Influencing Factors

doi:10.11924/j.issn.1000-6850.casb2024-0771

Abstract

Abstract:

In order to explore the accumulation dynamics of deoxynivalenol (DON) in wheat grain under field natural infected conditions and its correlation with disease spike rate, disease index, and percentage of fusarium damaged kernel, the disease spike rate, disease index, thousand grain weight, and percentage of fusarium damaged kernel of susceptible cultivar 'Huaimai 33' and moderately resistant cultivar 'Ningmai 13' from the early filling stage to the delayed harvest stage of 17 days and 15 days had been investigated by field natural infected in 2023 and 2024, respectively. Meanwhile, the DON content in grain had been detected by using ELISA method, and correlation analysis between the DON content in grain and disease spike rate, disease index, and percentage of fusarium damaged kernel had been conducted, respectively. The results showed that DON in grain had accumulated at the early filling stage before the appearance of fusarium head blight symptoms, and the DON content in grain were 237.99 and 182.95 ng/g, respectively. The DON in grain, disease spike rate, disease index, and percentage of fusarium damaged kernel gradually increased during the filling stage, showed slight increases during the dough stage, and tended to be stable during the complete ripe stage. No significant increases were observed during the delayed harvest stage, with no significant differences compared to the complete ripe stage. The thousand grain weight gradually increased during the filling stage, and had basically reached the maximum value by the end of the filling stage. For the same wheat cultivar, positive correlations were found between the DON content in grain and disease spike rate, disease index, and percentage of fusarium damaged kernel. The results of this study provided theoretical basis for the green prevention and control of fusarium head blight and DON in grain, as well as for reducing pesticide application, improving efficiency, and ensuring the quality and safety of agricultural products.

Key words: fusarium head blight, deoxynivalenol (DON), accumulation dynamic, disease index, fusarium damaged kernel

CHEN Hongzhou, ZHANG Haibo, ZHOU Chen, ZHU Youli, YANG Hongfu, XU Chao, GUO Xiaomeng, ZHU Feng. Wheat Grain Deoxynivalenol (DON) Accumulation Dynamics and the Correlation Analysis of Its Influencing Factors[J]. Chinese Agricultural Science Bulletin, 2025, 41(15): 7-14.

Figures/Tables 5

References 31

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编号	调查及取样时间(月/日)	生育期	病穗率/%	病情指数	千粒重/g	病粒率/%	DON/(ng/g)
1	4/29	灌浆期	0.00 h	0.00 i	6.27 i	0.00 e	237.99 de
2	5/2		0.75 h	0.11hi	10.45 h	0.00 e	181.90 e
3	5/5		2.83 g	0.47 h	14.10 g	0.00 e	158.71 e
4	5/8		4.99 f	0.91 g	21.83 f	2.00 de	442.31 cd
5	5/11		11.46 e	2.08 f	26.98 e	2.33 d	648.92 bc
6	5/14		18.62 d	3.39 e	30.12 d	2.67 cd	665.63 b
7	5/17		22.15 c	3.89 d	37.83 c	3.33 bcd	1464.55a
8	5/20		24.19 b	4.38 c	40.58 b	4.67 abc	1368.79 a
9	5/23		23.86 b	4.47 bc	43.88 a	5.33 ab	1440.68 a
10	5/26	蜡熟期	25.14 ab	4.62 abc	43.33 ab	3.33 bcd	1463.29 a
11	5/29	蜡熟期	25.79 a	4.88 ab	42.63 ab	4.00 abcd	1401.31 a
12	6/1	完熟期	26.18 a	4.74 abc	42.93 ab	4.67 abc	1339.24 a
13	6/4	延迟收获期	26.01 a	4.86 abc	43.27 ab	5.33 ab	1364.86 a
14	6/7		25.21 ab	4.65 abc	43.70 a	4.67 abc	1411.27 a
15	6/10		26.55 a	4.99 a	43.87 a	5.33 ab	1528.20 a
16	6/13		25.76 a	4.78 abc	43.65 a	6.00 a	1547.20 a
17	6/16		25.87 a	4.77 abc	43.53 a	4.67 abc	1389.27 a
18	6/19		26.32 a	4.91 ab	42.68 ab	5.33 ab	1542.53 a

编号	调查及取样时间(月/日)	生育期	病穗率/%	病情指数	千粒重/g	病粒率/%	DON/(ng/g)
1	4/29	灌浆期	0.00 h	0.00 i	6.27 i	0.00 e	237.99 de
2	5/2		0.75 h	0.11hi	10.45 h	0.00 e	181.90 e
3	5/5		2.83 g	0.47 h	14.10 g	0.00 e	158.71 e
4	5/8		4.99 f	0.91 g	21.83 f	2.00 de	442.31 cd
5	5/11		11.46 e	2.08 f	26.98 e	2.33 d	648.92 bc
6	5/14		18.62 d	3.39 e	30.12 d	2.67 cd	665.63 b
7	5/17		22.15 c	3.89 d	37.83 c	3.33 bcd	1464.55a
8	5/20		24.19 b	4.38 c	40.58 b	4.67 abc	1368.79 a
9	5/23		23.86 b	4.47 bc	43.88 a	5.33 ab	1440.68 a
10	5/26	蜡熟期	25.14 ab	4.62 abc	43.33 ab	3.33 bcd	1463.29 a
11	5/29	蜡熟期	25.79 a	4.88 ab	42.63 ab	4.00 abcd	1401.31 a
12	6/1	完熟期	26.18 a	4.74 abc	42.93 ab	4.67 abc	1339.24 a
13	6/4	延迟收获期	26.01 a	4.86 abc	43.27 ab	5.33 ab	1364.86 a
14	6/7		25.21 ab	4.65 abc	43.70 a	4.67 abc	1411.27 a
15	6/10		26.55 a	4.99 a	43.87 a	5.33 ab	1528.20 a
16	6/13		25.76 a	4.78 abc	43.65 a	6.00 a	1547.20 a
17	6/16		25.87 a	4.77 abc	43.53 a	4.67 abc	1389.27 a
18	6/19		26.32 a	4.91 ab	42.68 ab	5.33 ab	1542.53 a

编号	调查及取样时间(月/日)	生育期	病穗率/%	病情指数	千粒重/g	病粒率/%	DON/(ng/g)
1	4/26	灌浆期	0.00 h	0.00 g	15.82 l	0.00 e	182.95 g
2	4/29		0.32 gh	0.05 g	21.20 k	0.00 e	193.71 g
3	5/2		1.24 g	0.18 g	24.15 j	0.00 e	212.33 g
4	5/5		3.75 f	0.74 f	29.08 i	0.67 de	338.39 g
5	5/8		5.98 e	1.21 e	33.40 h	1.33 de	467.41 fg
6	5/11		9.59 d	1.96 d	35.88 g	2.00 cde	559.14 efg
7	5/14		11.51 c	2.53 c	37.65 f	2.67 bcd	733.70 def
8	5/17		12.35 bc	2.91 bc	38.97 e	3.67 abc	905.67 cde
9	5/20		13.20 ab	3.22 ab	40.18 cd	4.33 ab	963.99 bcd
10	5/23	蜡熟期	13.23 ab	3.29 ab	41.40 abcd	4.00 abc	992.17 abcd
11	5/26		13.38 ab	3.24 ab	42.18 a	5.00 a	1194.11 abc
12	5/29		13.78 a	3.44 a	41.53 abc	5.33 a	1113.85 abc
13	6/1	完熟期（收获期）	13.45 ab	3.31 ab	41.52 abc	4.00 abc	1091.53 abcd
14	6/4	延迟收获期	12.68 ab	3.04 ab	41.88 ab	3.67 abc	1260.86 abc
15	6/7		13.21 ab	3.21 ab	41.70 ab	4.67 ab	1318.86 ab
16	6/10		13.62 a	3.26 ab	40.64 bcd	5.00 a	1371.83 a
17	6/13		13.03 ab	3.13 ab	41.16 abcd	4.00 abc	1288.19 abc
18	6/16		13.82 a	3.39 a	40.72 bcd	4.67 ab	1149.95 abc

编号	调查及取样时间(月/日)	生育期	病穗率/%	病情指数	千粒重/g	病粒率/%	DON/(ng/g)
1	4/26	灌浆期	0.00 h	0.00 g	15.82 l	0.00 e	182.95 g
2	4/29		0.32 gh	0.05 g	21.20 k	0.00 e	193.71 g
3	5/2		1.24 g	0.18 g	24.15 j	0.00 e	212.33 g
4	5/5		3.75 f	0.74 f	29.08 i	0.67 de	338.39 g
5	5/8		5.98 e	1.21 e	33.40 h	1.33 de	467.41 fg
6	5/11		9.59 d	1.96 d	35.88 g	2.00 cde	559.14 efg
7	5/14		11.51 c	2.53 c	37.65 f	2.67 bcd	733.70 def
8	5/17		12.35 bc	2.91 bc	38.97 e	3.67 abc	905.67 cde
9	5/20		13.20 ab	3.22 ab	40.18 cd	4.33 ab	963.99 bcd
10	5/23	蜡熟期	13.23 ab	3.29 ab	41.40 abcd	4.00 abc	992.17 abcd
11	5/26		13.38 ab	3.24 ab	42.18 a	5.00 a	1194.11 abc
12	5/29		13.78 a	3.44 a	41.53 abc	5.33 a	1113.85 abc
13	6/1	完熟期（收获期）	13.45 ab	3.31 ab	41.52 abc	4.00 abc	1091.53 abcd
14	6/4	延迟收获期	12.68 ab	3.04 ab	41.88 ab	3.67 abc	1260.86 abc
15	6/7		13.21 ab	3.21 ab	41.70 ab	4.67 ab	1318.86 ab
16	6/10		13.62 a	3.26 ab	40.64 bcd	5.00 a	1371.83 a
17	6/13		13.03 ab	3.13 ab	41.16 abcd	4.00 abc	1288.19 abc
18	6/16		13.82 a	3.39 a	40.72 bcd	4.67 ab	1149.95 abc