Effects of Simulated Drought Stress on Seed Germination and Seedling Growth of Sophora alopecuroides

doi:10.11924/j.issn.1000-6850.casb2025-0247

Abstract

Abstract:

This study aims to investigate the effects of drought stress simulated by different concentrations of PEG-6000 (10%, 20%, 40%, 60%) on seed germination and seedling growth of Sophora alopecuroides. Using seeds and seedlings of S. alopecuroides as test materials, the experiment simulated drought conditions with the above-mentioned concentrations of PEG-6000, and determined and analyzed the morphological and physiological indices during seed germination and seedling growth. The results showed that at the seed germination stage, the PEG tolerance threshold for germination rate and radicle growth of S. alopecuroides seeds was 40%, while that for germination energy and germination index was 20%; after entering the seedling stage, the tolerance threshold of lateral roots and above-ground parts to PEG was 10%, and that of taproots and total biomass was 20%. Determination of physiological indices revealed that when PEG concentration was ≤40%, the contents of APX and Pro increased in a concentration-dependent manner, but decreased at 60%, whereas SOD content increased continuously with the increase of PEG concentration. In summary, PEG-simulated drought stress significantly inhibited seed germination and seedling growth of S. alopecuroides, with the seedling stage being more sensitive to stress; when PEG concentration was ≥40%, SOD played an active role in resisting physiological damage.

Key words: Sophora alopecuroides, PEG-simulated drought stress, seed germination, seedling growth, antioxidant enzymes, proline, morphological indices, osmotic stress

GAO Yingqi, LIU Bingbing. Effects of Simulated Drought Stress on Seed Germination and Seedling Growth of Sophora alopecuroides[J]. Chinese Agricultural Science Bulletin, 2025, 41(32): 82-87.

Figures/Tables 7

References 30

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PEG浓度/%	时间/d	侧根净生长量/mg	主根净生长量/mg	茎净生长量/mg
CK	3	+200±2.3^ab	+145±3.22^c	+281.5±2.48^d
CK	7	+400±4.5^d	+323±6.36^e	+412±4.92^f
10%	3	+110±3.22^a	+130±1.72^bc	+248±3.81^cd
10%	7	-233±3.17^b	+122±1.43^b	+292±3.10^d
20%	3	-247±4.33^bc	+120±1.92^b	-90±1.01^a
20%	7	-235±3.17^b	+88±0.78^a	-132±1.93^b
40%	3	-317±2.87^c	-92±0.89^ab	-217.4±2.81^c
40%	7	-378±3.56^cd	-143±1.28^c	-322±2.83^e
60%	3	-400±4.67^d	-238±1.39^d	-315.6±4.92^e
60%	7	-513±7.13^e	-455±2.73^f	-415.4±5.92^f

PEG浓度/%	时间/d	侧根净生长量/mg	主根净生长量/mg	茎净生长量/mg
CK	3	+200±2.3^ab	+145±3.22^c	+281.5±2.48^d
CK	7	+400±4.5^d	+323±6.36^e	+412±4.92^f
10%	3	+110±3.22^a	+130±1.72^bc	+248±3.81^cd
10%	7	-233±3.17^b	+122±1.43^b	+292±3.10^d
20%	3	-247±4.33^bc	+120±1.92^b	-90±1.01^a
20%	7	-235±3.17^b	+88±0.78^a	-132±1.93^b
40%	3	-317±2.87^c	-92±0.89^ab	-217.4±2.81^c
40%	7	-378±3.56^cd	-143±1.28^c	-322±2.83^e
60%	3	-400±4.67^d	-238±1.39^d	-315.6±4.92^e
60%	7	-513±7.13^e	-455±2.73^f	-415.4±5.92^f