Screening and Identification of High Efficiency Cellulose -degrading Bacteria for Garden Waste Under Natural Composting Conditions

doi:10.11924/j.issn.1000-6850.casb2025-0323

Abstract

Abstract:

This study aims to alleviate the accumulation pressure of urban garden waste, achieve the recycling of renewable resources, provide new approaches for the treatment of garden waste, and offer scientific support for the construction of a green and low-carbon waste treatment system. In this study, highly efficient cellulose-degrading strains were obtained from garden waste under natural fermentation and composting conditions, using sodium carboxymethyl cellulose as the sole carbon source. After screening, separation and purification, the strains were obtained. The initial screening was conducted by Congo red staining and filter paper strip disintegration, and the cellulase activity was determined by re-screening. The strains were identified by morphological and molecular biological identification methods. The research showed that after screening, isolation and purification, a cellulose-degrading fungus FH10 was obtained, and the enzyme activities of its filter paper, endoglucanase, exocytoglucanase and β-glucosylase were 0.5558 U/mL, 0.4916 U/mL, 1.107 U/mL and 1.663 U/mL, respectively. The FH10 strain was identified as Fusarium oxysporum by comparison with the NCBI-BLAST database. The FH10 strain is a highly efficient cellulose-degrading strain that can effectively promote the degradation of cellulose, laying a working foundation for the further development and utilization of microbial composting agents or enzyme preparations.

Key words: garden waste, cellulose degrading bacteria, screening, enzyme activity, identification

ZHENG Ping, LIU Jiali, HE Xin, WANG Yuxuan, DU Jie, MU Liqiang. Screening and Identification of High Efficiency Cellulose -degrading Bacteria for Garden Waste Under Natural Composting Conditions[J]. Chinese Agricultural Science Bulletin, 2025, 41(30): 90-96.

Figures/Tables 6

References 42

[1]	景海军. 城市建设中园林绿化体系的构建与优化[J]. 居舍, 2025(10):137-140.
[2]	刘娟. 低碳经济背景下对城市园林绿化建设的若干思考[J]. 城市建设理论研究(电子版), 2023(24):223-225.
[3]	厉桂香, 于田利, 牛超然, 等. 园林废弃物资源化利用及堆肥技术研究进展[J]. 现代园艺, 2023, 46(24):120-122.
[4]	梁晶, 方海兰, 严巍. 中国绿化植物废弃物资源化利用运营机制探讨[J]. 环境与可持续发展, 2016(5):55-59.
[5]	岳林芳, 苏少锋, 李蕴华, 等. 高效低温纤维素降解菌的筛选及其酶活特性[J]. 畜牧与饲料科学, 2025, 46(1):95-103.
[6]	马莲菊, 李龙, 羿晓庆, 等. 纤维素降解菌株性能优化及其资源化利用[J]. 沈阳师范大学学报(自然科学版), 2024, 42(6):514-520.
[7]	陈俊文, 刘庆华, 庞学勇. 一株厚壁隔孢伏革菌的筛选及木质纤维素降解特性[J]. 西南农业学报, 2023, 36(11):2491-2499.
[8]	杨毅, 杨善雅, 刘晓宇. 纤维素降解菌的筛选及鉴定[J]. 广东化工, 2025, 52(6):56-59.
[9]	李婷, 蒋朵, 卢映菲, 等. 园林废弃物高效降解菌的分离、筛选及鉴定[J]. 现代农业科技, 2022(5):125-128,135.
[10]	张梦君, 邱晨浩, 柴立伟, 等. 高效降解纤维素低温真菌的筛选、鉴定及发酵优化[J]. 微生物学通报, 2019, 46(10):2494-2503.
[11]	郭蔷, 郭薇, 李苗苗. 分析园林废弃物的生态处理及资源化利用[J]. 环境不发展, 2020, 32(8):76-77.
[12]	魏佳吉, 刘兴和, 朱兴盛, 等. 园林废弃物堆肥效果研究[J]. 林业科技情报, 2024, 56(1):25-29.
[13]	宋雨, 白红娟, 胡锦俊, 等. 秸秆纤维素降解菌的分离筛选、复合菌系构建及产酶条件优化[J]. 中国酿造, 2023, 42(2):108-114.
[14]	冯欣欣, 李凤兰, 徐永清, 等. 新疆寒冷地区腐木中产纤维素酶菌株的筛选与低温产酶特性[J]. 浙江农业学报, 2021, 33(8):1468-1476. doi: 10.3969/j.issn.1004-1524.2021.08.14
[15]	SHRIVASTAVA A, DASH D. Isolation, purification and characterization of cellulose-degrading bacteria from soil in Chhattisgarh, India[J]. Asian journal of soil science and plant nutrition, 2025, 11(1):323-327.
[16]	SHAMSHITOV A, SATKEVICIUTE E, DECOROSI F, et al. Phenotypic profiling of selected cellulolytic strains to develop a crop residue-decomposing bacterial consortium[J]. Microorganisms, 2025, 13(1):193-193.
[17]	陈莉. 绿色木霉固态发酵产纤维素酶条件研究[J]. 安徽农业科学, 2013, 41(7):2823-2825.
[18]	李晓秀, 张盼, 刘琬瑜, 等. 玉米秸秆降解真菌的筛选及鉴定[J]. 饲料工业, 2017, 38(3):37-42.
[19]	陈建爱, 贾梦莹, 陈为京. PDA中水含量对固体培养黄绿木霉T1010分生孢子生长发育的影响[J]. 中国农学通报, 2020, 558(15):112-120.
[20]	李正风, 李岩, 朱杰, 等. 烟草秸秆中产纤维素酶细菌筛选,鉴定及酶活测定[J]. 西南农业学报, 2020, 33(3):645-650.
[21]	杨娜, 何鑫, 杜春梅. 一株纤维素降解菌的筛选与鉴定[J]. 中国农学通报, 2021, 37(17):26-31. doi: 10.11924/j.issn.1000-6850.casb2021-0131
[22]	许玉林, 郑月霞, 叶冰莹, 等. 一株纤维素降解真菌的筛选及鉴定[J]. 微生物学通报, 2013, 40(2):220-227.
[23]	陈家楠. 纤维素结构研究的进展[J]. 纤维素科学与技术, 1993(4):1-10.
[24]	齐笑萱, 张阳, 赵爽, 等. 耐低温纤维素降解真菌的筛选、鉴定及酶活性研究[J]. 土壤通报, 2024, 55(2):460-470.
[25]	王海滨. 苦参提取残渣的堆肥化处理及防控土传病害研究[D]. 杨凌: 西北农林科技大学, 2015.
[26]	宁俊平, 孙如玉, 徐莹, 等. 纤维素降解菌研究综述[J]. 农家参谋, 2021(3):117-118.
[27]	王海滨, 韩立荣, 冯俊涛, 等. 高效纤维素降解菌的筛选及复合菌系的构建[J]. 农业生物技术学报, 2015, 23(4):421-431.
[28]	黄亚丽, 黄媛媛, 马慧媛, 等. 低温秸秆降解真菌的筛选及在秸秆还田中的应用[J]. 中国农学通报, 2020, 36(21):53-60. doi: 10.11924/j.issn.1000-6850.casb20200200102
[29]	SUN Y, QU J B, LI R L, et al. Optimization of the enzyme production conditions of Bacillus licheniformis and its effect on the degradation of corn straw[J]. Journal of biobased materials and bioenergy, 2018, 12(5):432-440.
[30]	龚小强, 孙向阳, 田赟, 等. 复合型有机改良剂对园林废弃物堆肥基质改良研究[J]. 西北林学院学报, 2013, 28(2):196-201.
[31]	张书瑜, 李霞. 园林绿化废弃物堆肥用于3种草花栽培基质的效果评价[J]. 现代园艺, 2022, 45(20):6-9.
[32]	郑燚李, 素艳, 向阳, 等. 园林废弃物制树穴覆盖板的应用性能[J]. 中国水土保持科学, 2020, 18(1):117-124.
[33]	王欣芸, 裴文影, 吴美满, 等. 不同园林废弃物生物炭去除水中CODMn, UV254的试验研究[J]. 广东化工, 2020, 47(21):98-100.
[34]	杨文敏. 黄孢原毛平革菌和康氏木霉联合降解园林废弃物及其应用[D]. 杭州: 浙江师范大学, 2021.
[35]	李文玉, 栾亚宁, 孙向阳, 等. 接种外源微生物菌剂对园林废弃物堆肥腐熟的影响[J]. 生态学杂志, 2014, 33(10):2670-2677.
[36]	ZHANG R, LU Q, ZHANG C, et al. Fungal pretreatment as a promising approach for simultaneous recovery of phosphorus and carbon resource from garden waste: performance and mechanism[J]. Water research x, 2025, 29(1)100330-100330.
[37]	ZOU J, LIU X, XU S, et al. Combined hydrothermal pretreatment of agricultural and forestry wastes to enhance anaerobic digestion for methane production[J]. Chemical engineering journal, 2024, 486(1):150313.
[38]	肖红. 绿化废弃物堆肥化处理模式和技术环节的探讨[J]. 中国园林, 2009, 25(4):7-11.
[39]	张家齐. 园林废弃物堆腐微生物过程及纤维素降解菌筛选研究[D]. 北京: 中国林业科学研究院, 2012.
[40]	付冰妍, 孙向阳, 余克非, 等. 降解园林废弃物专用固体复合菌的构建及其堆肥效应研究[J]. 环境科学研究, 2021, 34(5):1231-1237.
[41]	GAO X J, ZHANG H T, QIAN C R, et al. Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency[J]. Annals of microbiology, 2020, 70(1):1021-1242.
[42]	BRTNICKY M, PECINA V, KUCERIK J, et al. Biodegradation of poly-3-hydroxybutyrate after soil inoculation with microbial consortium: soil microbiome and plant responses to the changed environment[J]. The science of the total environment, 2024, 946(1):174328.

菌株编号	透明圈直径/mm	菌落直径/mm	透明圈直径/ 菌落直径
XW2	7.30±0.35	3.80±0.21	1.9211 de
XW3	5.20±0.41	1.80±0.15	2.8889 ab
XJ2	7.30±0.28	3.70±0.19	1.9211 de
XJ7	2.30±0.26	0.98±0.08	2.3470 cd
XJ12	2.90±0.31	1.20±0.11	2.4167 bcd
XJ18	5.30±0.39	3.20±0.25	1.6563 e
XJ20	4.90±0.22	1.90±0.16	2.5789 abc
XJ22	3.80±0.33	1.50±0.14	2.5333 bc
FH3	7.20±0.45	6.80±0.38	1.0588 f
FH10	3.90±0.29	1.30±0.12	3.2500 a

菌株编号	透明圈直径/mm	菌落直径/mm	透明圈直径/ 菌落直径
XW2	7.30±0.35	3.80±0.21	1.9211 de
XW3	5.20±0.41	1.80±0.15	2.8889 ab
XJ2	7.30±0.28	3.70±0.19	1.9211 de
XJ7	2.30±0.26	0.98±0.08	2.3470 cd
XJ12	2.90±0.31	1.20±0.11	2.4167 bcd
XJ18	5.30±0.39	3.20±0.25	1.6563 e
XJ20	4.90±0.22	1.90±0.16	2.5789 abc
XJ22	3.80±0.33	1.50±0.14	2.5333 bc
FH3	7.20±0.45	6.80±0.38	1.0588 f
FH10	3.90±0.29	1.30±0.12	3.2500 a

编号	培养时间
编号	1 d	2 d	3 d	4 d	5 d	6 d	7 d	8 d	9 d	10 d	11 d	12 d	13 d	14 d
XW2	+	+	+	+	+	++	++	++	+++	+++	+++	+++	++++
XJ2	+	+	+	+	++	++	++	++	+++	+++	+++	+++	+++	++++
XJ7		+	+	++	++	++	++	+++	+++	+++	+++	+++	++++	++++
XJ12	+	++	++	++	++	++	++	++	+++	+++	+++	+++	++++
XJ18		+	++	++	++	++	+++	+++	+++	++++
XJ20	+	+	+	+	++	++	++	+++	+++	+++	++++
FH3		+	+	++	++	++	++	+++	+++	+++	+++	++++
FH10		+	++	++	+++	+++	+++	+++	++++

编号	培养时间
编号	1 d	2 d	3 d	4 d	5 d	6 d	7 d	8 d	9 d	10 d	11 d	12 d	13 d	14 d
XW2	+	+	+	+	+	++	++	++	+++	+++	+++	+++	++++
XJ2	+	+	+	+	++	++	++	++	+++	+++	+++	+++	+++	++++
XJ7		+	+	++	++	++	++	+++	+++	+++	+++	+++	++++	++++
XJ12	+	++	++	++	++	++	++	++	+++	+++	+++	+++	++++
XJ18		+	++	++	++	++	+++	+++	+++	++++
XJ20	+	+	+	+	++	++	++	+++	+++	+++	++++
FH3		+	+	++	++	++	++	+++	+++	+++	+++	++++
FH10		+	++	++	+++	+++	+++	+++	++++