Effects of Extreme Rainstorms on Mycorrhizae of Alien and Native Plants in Abandoned Karst Farmlands

doi:10.11924/j.issn.1000-6850.casb2025-0375

Abstract

Abstract:

This study aimed to explore the responses of mycorrhizae of alien plants and native plants to the changes of rainstorm frequency in karst areas. Dominant alien plants and native plants from karst abandoned land under four rainfall treatments (natural rainfall (CK), low-frequency rainstorm (T20), medium-frequency rainstorm (T40), and high-frequency rainstorm (T60)) were used to determine the mycorrhizal colonization rate of plants, soil hyphal density, and spore density. The results showed: (1) Medium-frequency and high-frequency rainstorm treatments significantly increased the arbuscular colonization rate of native plants (P<0.05) and improved soil spore density, but decreased the arbuscular colonization rate of alien plants. Among them, the spore density of alien plants only increased under high-frequency rainstorm treatment. (2) Medium-frequency rainstorm treatment significantly reduced the hyphal density of native plants (P<0.05), while the hyphal density of alien plants showed no significant change. (3) Extreme rainstorm treatments reduced the number of plant species with mycorrhizal differences, and different plant species showed variations in their responses to extreme rainstorms. The results indicated that rainstorm frequency affected the mycorrhizal colonization characteristics differently for different plant species; native plants exhibited a more sensitive response to rainfall changes.

Key words: extreme rainstorm, alien plant, native plant, mycorrhizal colonization rate, hyphal density, spore density, arbuscular mycorrhizal fungi, Karst

CLC Number:

S181

ZHAN Yifei, ZHAO Yonglin, QI Yuancai, TAO Jianping. Effects of Extreme Rainstorms on Mycorrhizae of Alien and Native Plants in Abandoned Karst Farmlands[J]. Chinese Agricultural Science Bulletin, 2026, 42(9): 107-116.

Figures/Tables 10

References 52

[1]	赵光华, 高明龙, 王朵, 等. 全球入侵植物的经济成本评估[J]. 草业学报, 2024, 33(5):16-24. doi: 10.11686/cyxb2023220
[2]	REINHART K O, CALLAWAY R M. Soil biota and invasive plants[J]. The new phytologist, 2006, 170(3):445-457. doi: 10.1111/nph.2006.170.issue-3 URL
[3]	刘艳杰, 黄伟, 杨强, 等. 近十年植物入侵生态学重要研究进展[J]. 生物多样性, 2022, 30(10):272-288.
[4]	马路平, 石兆勇, 张梦歌, 等. 中国植物丛枝菌根侵染特征研究[J]. 微生物学报, 2024, 64(7):2566-2582.
[5]	FREW A, POWELL J R, JOHNSON S N. Aboveground resource allocation in response to root herbivory as affected by the arbuscular mycorrhizal symbiosis[J]. Plant and soil, 2020, 447(1-2):463-473. doi: 10.1007/s11104-019-04399-x
[6]	ZHANG F, LI Q, CHEN F, et al. Arbuscular mycorrhizal fungi facilitate growth and competitive ability of an exotic species Flaveria bidentis[J]. Soil biology & biochemistry, 2017, 115:275-284. doi: 10.1016/j.soilbio.2017.08.019 URL
[7]	AWAGUL A, WANYING Z, YONGGE Y, et al. Common mycorrhizal networks influence the distribution of mineral nutrients between an invasive plant, Solidago canadensis, and a native plant, Kummerowa striata[J]. Mycorrhiza, 2019, 29(1):29-38. doi: 10.1007/s00572-018-0873-5
[8]	WALLER L P, CALLAWAY R M, KLIRONOMOS J N, et al. Reduced mycorrhizal responsiveness leads to increased competitive tolerance in an invasive exotic plant[J]. Journal of ecology, 2016, 104(6):1599-1607. doi: 10.1111/jec.2016.104.issue-6 URL
[9]	GILLESPIE I G, ALLEN E B. Effects of soil and mycorrhizae from native and invaded vegetation on a rare California forb[J]. Applied soil ecology, 2006, 32(1):6-12. doi: 10.1016/j.apsoil.2005.03.008 URL
[10]	丁波, 贺新春, 沈代欣. 遵义市中心城区极端暴雨遭遇频率研究[J]. 水资源开发与管理, 2025, 11(8):56-63.
[11]	刘鑫, 李思亮, 岳甫均, 等. 喀斯特系统生物地球化学循环及对全球变化的响应[J]. 中国岩溶, 2022, 41(3):465-476.
[12]	ZHANG L, ZHOU J, GEORGE T S, et al. Arbuscular mycorrhizal fungi conducting the hyphosphere bacterial orchestra[J]. Trends in plant science, 2021, 27(4):402-411. doi: 10.1016/j.tplants.2021.10.008 pmid: 34782247
[13]	FU W, CHEN B, RILLIG M C, et al. Community response of arbuscular mycorrhizal fungi to extreme drought in a cold-temperate grassland[J]. The new phytologist, 2021, 234(6):2003-2017. doi: 10.1111/nph.v234.6 URL
[14]	DEVEAUTOUR C, POWER S A, BARNETT K L, et al. Temporal dynamics of mycorrhizal fungal communities and co-associations with grassland plant communities following experimental manipulation of rainfall[J]. Journal of ecology, 2020, 108(2):515-527. doi: 10.1111/jec.v108.2 URL
[15]	FERNANDEZ C W, MIELKE L, STEFANSKI A, et al. Climate change-induced stress disrupts ectomycorrhizal interaction networks at the boreal-temperate ecotone[J]. Proceedings of the national academy of sciences, 2023, 120(34):e2073348176.
[16]	XIE T, LIN Y, LI X. Responses of the arbuscular mycorrhizal fungi community to warming coupled with increased drought in an arid desert region[J]. Geoderma, 2024, 441:116744. doi: 10.1016/j.geoderma.2023.116744 URL
[17]	CHEN E, LIAO H, CHEN B, et al. Arbuscular mycorrhizal fungi are a double-edged sword in plant invasion controlled by phosphorus concentration[J]. New phytologist, 2020, 226(2):295-300. doi: 10.1111/nph.16359 pmid: 31808168
[18]	TIAN B, PEI Y, LI W, et al. Gene expression controlling signalling molecules within mutualistic associations of an invasive plant: an evolutionary perspective[J]. Journal of ecology, 2024, 112(8):1818-1831. doi: 10.1111/jec.v112.8 URL
[19]	梁倩倩, 李敏, 刘润进, 等. 全球变化下菌根真菌的作用及其作用机制[J]. 生态学报, 2014, 34(21):6039-6048.
[20]	国家环境保护总局, 中国科学院.关于发布中国第一批外来入侵物种名单的通知[EB/OL]. https://www.mee.gov.cn/gkml/zj/wj/200910/t20091022_172155.htm, 2003-01-10/2025-07-20.
[21]	环境保护部, 中国科学院. 关于发布《中国外来入侵物种名单(第三批)》的公告[EB/OL]. https://www.mee.gov.cn/gkml/hbb/bgg/201408/t20140828_288367.htm, 2014-08-20/2025-11-14.
[22]	马金双. 中国入侵植物名录[M]. 北京: 高等教育出版社, 2013:1-324.
[23]	王思雨, 魏涵, 陈科宇, 等. 丛枝菌根真菌(AMF)孢子、菌丝密度及侵染率定量测定方法[EB/OL]. https://doi.org/10.21769/BioProtoc.2104253, 2022-01-01/2025-11-14.
[24]	王晓英, 王冬梅, 陈保冬, 等. 丛枝菌根真菌群落对白三叶草生长的影响[J]. 生态学报, 2010, 30(6):1456-1462.
[25]	毕银丽, 吴王燕. 染色法——一种改进的丛枝菌根孢子密度快速测定方法[J]. 能源环境保护, 2007(2):9-11.
[26]	毕银丽, 张可, 肖礼, 等. 矿区复垦地接菌驱动植物-土壤系统中光合碳分配与稳定机制[J]. 煤炭学报, 2025, 50(1):572-583.
[27]	岳辉, 毕银丽. 基于野外大田试验的接菌紫穗槐生态修复效应研究[J]. 水土保持通报, 2017, 37(4):1-5.
[28]	LENOIR I, FONTAINE J, SAHRAOUI A L. Arbuscular mycorrhizal fungal responses to abiotic stresses: a review[J]. Phytochemistry, 2016, 123:4-15. doi: 10.1016/j.phytochem.2016.01.002 pmid: 26803396
[29]	韩金吉, 沈小奥, 杨帆, 等. 丛枝菌根真菌(AMF)介导植物矿质元素吸收机制的研究进展[J]. 草地学报, 2023, 31(6):1609-1621. doi: 10.11733/j.issn.1007-0435.2023.06.002
[30]	SOKA G E, RITCHIE M E. Arbuscular mycorrhizal spore composition and diversity associated with different land uses in a tropical savanna landscape, Tanzania[J]. Applied soil ecology, 2018, 125:222-232. doi: 10.1016/j.apsoil.2018.01.013 URL
[31]	AGUILERA L E, ARMAS C, CEA A P, et al. Rainfall, microhabitat, and small mammals influence the abundance and distribution of soil microorganisms in a Chilean semi-arid shrubland[J]. Journal of arid environments, 2016, 126:37-46. doi: 10.1016/j.jaridenv.2015.11.013 URL
[32]	曹聪. 隔离降雨和氮沉降对杉木人工林丛枝菌根真菌与细根的影响[D]. 福州: 福建师范大学, 2021:35-44.
[33]	FABIO B, METTE G, MONICA A, et al. Facilitation of phosphorus uptake in maize plants by mycorrhizosphere bacteria[J]. Scientific reports, 2017, 7(1-4):4686. doi: 10.1038/s41598-016-0028-x
[34]	段海霞, 罗崇亮, 师茜, 等. 丛枝菌根真菌对植物-土壤系统的影响研究进展[J]. 生态学报, 2025, 45(1):475-491.
[35]	金小霞. AMF根外菌丝对铅的吸收转运及固定作用[D]. 杨凌: 西北农林科技大学, 2022:21-26.
[36]	LE PIOUFLE O, DECLERCK S. Reducing water availability impacts the development of the arbuscular mycorrhizal fungus rhizophagus irregularis MUCL 41833 and its ability to take up and transport phosphorus under in vitro conditions[J]. Frontiers in microbiology, 2018, 9:1254. doi: 10.3389/fmicb.2018.01254 URL
[37]	孙向伟, 王晓娟, 陈牧, 等. 生态环境因子对AM真菌孢子形成与分布的作用机制[J]. 草业学报, 2011, 20(1):214-221.
[38]	GIOVANNETTI M, AVIO L, SBRANA C. Fungal spore germination and pre-symbiotic mycelial growth-physiological and genetic aspects. In: KoltaiH, KapulnikY. Arbuscular Mycorrhizas: Physiology and Function[M]. Dordrecht: Springer Netherlands, 2010:3-32.
[39]	DAVID R, KURT I, OLIVIER B, et al. Bacteria associated with spores of the arbuscular mycorrhizal fungi Glomus geosporum and Glomus constrictum[J]. Applied and environmental microbiology, 2005, 71(11):6673-6679. doi: 10.1128/AEM.71.11.6673-6679.2005 URL
[40]	巩晓芳, 祝英, 彭轶楠, 等. 当归不同生长时期根际丛枝真菌分布及土壤养分和酶活性的动态变化[J]. 微生物学通报, 2017, 44(11):2596-2605.
[41]	黄玉丹, 张淑彬, 李琳, 等. 丛枝菌根真菌繁殖体的高效扩繁[J]. 微生物学通报, 2023, 50(2):503-513.
[42]	季生连, 杨沐, 段国珍, 等. 基于Illumina MiSeq技术分析盐生植物根围AM真菌定殖和群落组成[J/OL]. 中国农业科技导报,https://doi.org/10.13304/j.nykjdb.2024.0723, 2024-11-29/2025-11-14.
[43]	曾凯, 张欣然, 刘赛博, 等. 9种蕨类植物丛枝菌根真菌侵染的结构观察[J]. 西北植物学报, 2023, 43(5):772-780.
[44]	王健, 李娟, 卢垟杰. 毛乌素沙地不同植物AM真菌菌丝侵染特性比较研究[A]. 2019年全国学术年会论文集(下册)[C]. 北京: 工业建筑杂志社有限公司, 2019:977-980.
[45]	李军帅. 丛枝菌根真菌菌丝侵染特性与植物系统性关系的研究[D]. 兰州: 兰州大学,2016:1-3.
[46]	PORPORATO A, VICO G, FAY P A. Superstatistics of hydro-climatic fluctuations and interannual ecosystem productivity[J]. Geophysical research letters, 2006, 33(15):15401-15402.
[47]	祝英, 熊俊兰, 吕广超, 等. 丛枝菌根真菌与植物共生对植物水分关系的影响及机理[J]. 生态学报, 2015, 35(8):2419-2427.
[48]	常学礼, 赵爱芬, 李胜功. 科尔沁沙地固定沙丘植被物种多样性对降水变化的响应[J]. 植物生态学报, 2000(2):147-151.
[49]	刘军. 果园生草的优良草种——蛇莓[J]. 江西农业, 2016(23):73-79.
[50]	刘黎君. 对陇东黄土高原区优良水土保持灌木茅莓的研究[J]. 甘肃农业, 2013(6):29-31.
[51]	陈珂. 蛇莓等四种地被植物抗旱性研究[D]. 北京: 北京林业大学,2008:43-44.
[52]	昝瑛, 索朗玉珍, 马竟文, 等. 降雨极端化对喀斯特草本植物功能性状的影响[J]. 西南大学学报(自然科学版), 2022, 44(9):20-31.

分组	序号	物种名	科名	生活型
本地植物	1	狗尾草(Setaria viridis)	禾本科	一年生草本
	2	柔枝莠竹(Microstegium vimineum)	禾本科	一年生草本
	3	茅莓(Rubus parvifolius)	蔷薇科	灌木
	4	蛇莓(Duchesnea indica)	蔷薇科	多年生草本
	5	杠板归(Persicaria perfoliata)	蓼科	一年生草本
	6	香附子(Cyperus rotundus)	莎草科	多年生草本
外来植物	7	小蓬草(Erigeron canadensis)	菊科	一年生草本
	8	喜旱莲子草(Alternanthera philoxeroides)	苋科	一年生草本
	9	野胡萝卜(Daucus carota)	伞形科	一年生草本
	10	红花酢浆草(Oxalis corymbosa)	酢浆草科	多年生草本

分组	序号	物种名	科名	生活型
本地植物	1	狗尾草(Setaria viridis)	禾本科	一年生草本
	2	柔枝莠竹(Microstegium vimineum)	禾本科	一年生草本
	3	茅莓(Rubus parvifolius)	蔷薇科	灌木
	4	蛇莓(Duchesnea indica)	蔷薇科	多年生草本
	5	杠板归(Persicaria perfoliata)	蓼科	一年生草本
	6	香附子(Cyperus rotundus)	莎草科	多年生草本
外来植物	7	小蓬草(Erigeron canadensis)	菊科	一年生草本
	8	喜旱莲子草(Alternanthera philoxeroides)	苋科	一年生草本
	9	野胡萝卜(Daucus carota)	伞形科	一年生草本
	10	红花酢浆草(Oxalis corymbosa)	酢浆草科	多年生草本

项目	DF	总侵染率		丛枝侵染率		泡囊侵染率		菌丝侵染率
项目	DF	F值	P值	F值	P值	F值	P值	F值	P值
物种	9	15.500	**	6.998	**	6.355	**	5.163	**
降雨处理	3	0.565	0.640	4.424	**	3.199	*	0.887	0.452
降雨处理×物种	27	2.539	**	1.236	0.232	1.742	*	1.236	0.232

项目	DF	总侵染率		丛枝侵染率		泡囊侵染率		菌丝侵染率
项目	DF	F值	P值	F值	P值	F值	P值	F值	P值
物种	9	15.500	**	6.998	**	6.355	**	5.163	**
降雨处理	3	0.565	0.640	4.424	**	3.199	*	0.887	0.452
降雨处理×物种	27	2.539	**	1.236	0.232	1.742	*	1.236	0.232

物种	CK	差异性	T20	差异性	T40	差异性	T60	差异性
狗尾草	0.02±0.02	Aa	0.04±0.03	Aa	0.02±0.04	Aa	0.07±0.03	Aa
柔枝莠竹	0.06±0.06	Aa	0.01±0.03	Aa	0.07±0.04	Aa	0.09±0.05	Aa
茅莓	0.02±0.02	Aa	0.02±0.02	Aa	0.01±0.01	Aa	0.04±0.03	Aa
蛇莓	0.01±0.02	Ba	0.01±0.01	Ba	0.23±0.10	Aa	0.10±0.03	ABa
杠板归	0.02±0.03	Aa	0.01±0.01	Aa	0.02±0.04	Aa	0.00±0.01	Aa
香附子	0.00±0.00	Aa	0.00±0.00	Aa	0.04±0.04	Aa	0.05±0.06	Aa
小蓬草	0.20±0.13	Aa	0.21±0.11	Aa	0.17±0.20	Aa	0.15±0.10	Aa
喜旱莲子草	0.00±0.00	Aa	0.02±0.02	Aa	0.01±0.01	Aa	0.01±0.01	Aa
野胡萝卜	0.00±0.00	Aa	0.01±0.01	Aa	0.01±0.02	Aa	0.04±0.06	Aa
红花酢浆草	0.00±0.01	Aa	0.01±0.01	Aa	0.02±0.03	Aa	0.01±0.01	Aa