Effects of Continuous Planting of Transgenic Maize on Community of Arbuscular Mycorrhizal Fungi in Soil

doi:10.11924/j.issn.1000-6850.casb2024-0062

Abstract

Abstract:

To evaluate the effect of continuous planting of transgenic maize on the community structure of soil arbuscular mycorrhizal fungi, transgenic maize DBN9936 and its non-transgenic counterpart were used as the experimental materials, the characteristics of soil arbuscular mycorrhizal (AM) fungal community structure were analyzed using PCR-DGGE and PLFA techniques. The results indicated that there was a high similarity in the soil AM fungal community structure between transgenic and non-transgenic maize in the same planting year and location. There were no significant differences in the Shannon-Wiener index, Pielou evenness index and richness. The dominant genera consistently identified were Glomus. Cluster analysis revealed that the similarity of AM fungal communities in soils from the two maize types at the same planting location was mostly above 0.60, while the similarity between communities from different locations was below 0.60. Additionally, the clustering pattern clearly separated the two maize types from different planting locations on the upper and lower sides of the cluster diagram, indicated that the community structure of soil AM fungi was greatly affected by planting sites, while the influence of the transgenic itself was weak. Phylogenetic analysis also indicated that the specific bands of the two maize species planted in different locations clustered in different groups on the phylogenetic trees. The results of soil microbial PLFA content showed that the planting of transgenic maize DBN9936 had no significant effect on the total soil microbial PLFA content, and the differences in the relative abundance of microbial groups were small. In contrast, significant differences were observed in the total PLFA content of soil microbes between the two maize types planted at different locations, with substantial variations in the relative abundance of microbial groups. In conclusion, compared to non-transgenic maize, transgenic maize DBN9936 had no significant impact on the structure of soil AM fungal communities and PLFA content. However, notable differences were observed among different planting locations.

Key words: transgenic maize, arbuscular mycorrhizal fungi, PCR-DGGE, PLFA, community structure

LIU Qianlong, LIU Ruihua, LI Gang, XIU Weiming, YANG Dianlin, LIU Hongmei, ZHAO Jianning. Effects of Continuous Planting of Transgenic Maize on Community of Arbuscular Mycorrhizal Fungi in Soil[J]. Chinese Agricultural Science Bulletin, 2024, 40(29): 65-74.

Figures/Tables 12

References 37

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试验地	全磷/(g/kg)	全氮/(g/kg)	土壤有机质/(g/kg)	pH
吉林	0.52	1.02	9.35	5.16
河北	0.53	0.49	9.35	8.37

试验地	全磷/(g/kg)	全氮/(g/kg)	土壤有机质/(g/kg)	pH
吉林	0.52	1.02	9.35	5.16
河北	0.53	0.49	9.35	8.37

巢式PCR	引物	引物序列(5′-3′)	反应条件
第一步	AML1	ATCAACTTTCGATGGTAGGATAGA	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min
第一步	AML2	GAACCCAAACACTTTGGTTTCC	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min
第二步	NS31	GC-CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCA CGGGGGGTTGGA	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min
第二步	G101	GGGCAA GTCTGGTGCC GCC TGC TTT AAA CAC TCT A	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min

巢式PCR	引物	引物序列(5′-3′)	反应条件
第一步	AML1	ATCAACTTTCGATGGTAGGATAGA	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min
第一步	AML2	GAACCCAAACACTTTGGTTTCC	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min
第二步	NS31	GC-CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCA CGGGGGGTTGGA	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min
第二步	G101	GGGCAA GTCTGGTGCC GCC TGC TTT AAA CAC TCT A	94℃ 3 min；94℃ 30 s，50℃ 30 s， 72℃ 45 s，35个循环；72℃ 7 min

微生物类群	磷脂脂肪酸标记
细菌	i14:0，14:0，i15:0，a15:0，15:0，i16:0，16:0，16:1ω7c，i17:0，a17:0，17:0，cy17:0，18:1ω7，i19:0，a19:0，19:0，cy19:0
革兰氏阳性细菌	i14:0，i15:0，a15:0，i16:0，i17:0，a17:0
革兰氏阴性细菌	16:1ω7c，16:1ω9c，cyc17:0，17:1ω8c，18:1ω7c，cyc19:0
真菌	16:1ω5c，18:1ω9c，18:2ω6c，18:3ω6c
AM真菌	16:1ω5c，18:1ω7
厌氧菌	A17:0，i17:0