Effect of Entomopathogenic Nematode Combining with Soil Amendment on Soil Nematode Community Structure in Rhizosphere of Cucumber

doi:10.11924/j.issn.1000-6850.casb2023-0693

Abstract

Abstract:

To investigate the inhibitory effect of soil amendment (SA) in combination with entomopathogenic nematode (Heterorhabditis beicherriana) on Meloidogyne incognita, and its impact on the ecological environment of cucumber rhizosphere soil, we used the cucumber cultivar ‘Qianqiu No.1’ as the test variety, and set up six different experimental groups to study the community structure of soil nematode, ecological diversity and functional diversity of cucumber rhizosphere soil. The results showed that after 60 days of experiment, the SA and nematode H. beicherriana group contained fewer parasitic nematodes in root nodules, and more Rhabditis and Mesorhabditis nematodes. The trophic diversity and basal index were increased, and the nematode channel ratio was significantly increased, and the soil organic decomposition was mainly bacterial decomposition. The nematode population remained predominantly in area A, characterized by better soil nutrients and bacterial decomposition, whereas in other groups, there was a gradual decrease from area A to area D. The results showed that the SA soil restorer combined with entomopathogenic nematode (H. beicherriana) could effectively inhibit M. incongnita, and at the same time improve the diversity of soil nematodes, effectively alleviate the degradation trend of soil food web.

Key words: soil amendment, entomopathogenic nematode, Meloidogyne incognita, cucumber, community structure

FU Huijuan, ZHANG Haiting, YI Jun, ZENG Shuocheng, XU Bingzhi, YE Jianrong, LI Qiyong, ZHANG Hong, LI Xingyue. Effect of Entomopathogenic Nematode Combining with Soil Amendment on Soil Nematode Community Structure in Rhizosphere of Cucumber[J]. Chinese Agricultural Science Bulletin, 2024, 40(28): 134-142.

Figures/Tables 6

References 34

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编号	处理	根结线虫J₂/条	昆虫病原线虫J₃/条	SA原液/mL
CK	清水对照组	0	0	0
M	根结线虫对照组	300	0	0
M+SA	SA处理组	300	0	0.4
M+H	EPN处理组	300	300	0
H+SA	SA与EPN对照组	0	300	0.4
M+SA+H	SA与EPN处理	300	300	0.4

编号	处理	根结线虫J₂/条	昆虫病原线虫J₃/条	SA原液/mL
CK	清水对照组	0	0	0
M	根结线虫对照组	300	0	0
M+SA	SA处理组	300	0	0.4
M+H	EPN处理组	300	300	0
H+SA	SA与EPN对照组	0	300	0.4
M+SA+H	SA与EPN处理	300	300	0.4