Studies on Effects of Nitrate Nitrogen and Ammonium Nitrogen Combination on Alleviating Ammonium Toxicity in Cucumber Seedlings

doi:10.11924/j.issn.1000-6850.casb2023-0213

Abstract

Abstract:

To explore the effects of NO₃^--N on alleviating ammonium toxicity and mechanism involved at cucumber seedling stage, a pot experiment was carried out. In the experiment, the variety of ‘Jinyan No.4’ was selected as the experimental material, and vermiculite was used as the seedling substrate. The total nitrogen concentration was 15 mmol/L, NH₄⁺-N and NO₃^--N were supplied respectively at seedling stage. 5 weeks later, the growth and physiological indexes of cucumber seedlings were measured. The results showed that it was easy to cause toxicity, if only NH₄⁺-N was supplied. The contents of total nitrogen and total phosphorus in shoots treated by NO₃^--N were 1.08 times and 1.11 times higher than those treated by NH₄⁺-N, respectively. Meanwhile, the leaf area, biomass and seedling's quality index were significantly higher than those treated by NH₄⁺-N. For seedlings only supplied with NH₄⁺-N, MDA content in shoots was significantly higher than that of NO₃^--N treatment, but chlorophyll content was significantly lower than that of NO₃^--N treatment. The growth was significantly inhibited by NH₄⁺-N. When NH₄⁺-N was mixed with the same amount of NO₃^--N, the inhibition was weakened. The pH value of seedlings rhizosphere increased from 6.28 to 6.54, at the same time chlorophyll content and LA increased in a degree. The shoots and roots dry weights increased by 26.6% and 29.5%, respectively. There was no significant difference in seedling's quality index between NH₄⁺-N treatment and NO₃^--N treatment. It is concluded that ammonium toxicity is relieved by NO₃^--N, and the beneficial effect depends on the balance of nutrition and physiological metabolism of seedlings.

Key words: cucumber, NH₄⁺-N, NO₃^--N, ammonium toxicity, alleviating

FAN Yaping, LI Jingyi, HE Miaomiao, WANG Changxian. Studies on Effects of Nitrate Nitrogen and Ammonium Nitrogen Combination on Alleviating Ammonium Toxicity in Cucumber Seedlings[J]. Chinese Agricultural Science Bulletin, 2024, 40(7): 27-32.

Figures/Tables 9

References 34

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处理	株高/×10^-2 m	茎粗/×10^-2 m	叶面积/×10^-4 m²	生物量/(g/盆)		壮苗指数
处理	株高/×10^-2 m	茎粗/×10^-2 m	叶面积/×10^-4 m²	地上部	根系	壮苗指数
NN	9.22±0.59	0.47±0.01a	285.40±31.24a	0.7488±0.06a	0.1418±0.01a	13.15±2.28a
1/2NN+1/2AN	7.50±0.40	0.46±0.03a	216.91±3.70b	0.6885±0.06ab	0.1337±0.01ab	11.12±1.37a
AN	9.62±1.15	0.38±0.02b	178.35±13.82b	0.5440±0.03b	0.1032±0.01b	4.78±0.72b

处理	株高/×10^-2 m	茎粗/×10^-2 m	叶面积/×10^-4 m²	生物量/(g/盆)		壮苗指数
处理	株高/×10^-2 m	茎粗/×10^-2 m	叶面积/×10^-4 m²	地上部	根系	壮苗指数
NN	9.22±0.59	0.47±0.01a	285.40±31.24a	0.7488±0.06a	0.1418±0.01a	13.15±2.28a
1/2NN+1/2AN	7.50±0.40	0.46±0.03a	216.91±3.70b	0.6885±0.06ab	0.1337±0.01ab	11.12±1.37a
AN	9.62±1.15	0.38±0.02b	178.35±13.82b	0.5440±0.03b	0.1032±0.01b	4.78±0.72b

处理	地上部			根系
处理	含量/%	吸收量/mg	利用效率	含量/%	吸收量/mg	利用效率
NO₃^--N	5.82±0.40	43.62±4.64a	17.44±1.33	3.56±0.05	5.06±0.27	28.10±0.38
1/2NN+1/2AN	5.05±0.32	34.52±2.94ab	20.06±1.39	4.79±0.68	6.28±0.88	22.25±3.25
NH₄⁺-N	5.41±0.15	29.39±1.82b	18.52±0.55	5.49±0.95	5.38±0.49	20.28±4.14

处理	地上部			根系
处理	含量/%	吸收量/mg	利用效率	含量/%	吸收量/mg	利用效率
NO₃^--N	5.82±0.40	43.62±4.64a	17.44±1.33	3.56±0.05	5.06±0.27	28.10±0.38
1/2NN+1/2AN	5.05±0.32	34.52±2.94ab	20.06±1.39	4.79±0.68	6.28±0.88	22.25±3.25
NH₄⁺-N	5.41±0.15	29.39±1.82b	18.52±0.55	5.49±0.95	5.38±0.49	20.28±4.14

处理	地上部			根系
处理	含量/%	吸收量/mg	利用效率	含量/%	吸收量/mg	利用效率
NO₃^--N	0.43±0.01a	3.20±0.27a	234.29±3.55b	0.37±0.03	0.52±0.03a	277.92±21.15
1/2NN+1/2AN	0.37±0.02b	2.52±0.15b	273.24±18.31a	0.34±0.01	0.46±0.04ab	290.60±8.62
NH₄⁺-N	0.39±0.01ab	2.09±0.10b	260.52±8.58ab	0.37±0.05	0.37±0.03b	282.73±38.17