Identification and Mitochondrial Genome Analysis of a New Stored Pest of Sweetpotato: Paralipsa gularis (Zeller)

doi:10.11924/j.issn.1000-6850.casb2025-0603

Abstract

Abstract:

This study aims to provide basic information on the new storage pest of sweet potatoes, Paralipsa gularis (Zeller), clarifying its phylogenetic relationship within the order Lepidoptera. This lays the basic for the identification and control of this pest. The author identified the morphological characteristics and cox1 gene of the pest, and utilized Illumina MiSeq sequencing technology to sequence its mitochondrial genome. The structural features and base composition were analyzed, and a phylogenetic tree was constructed using the maximum likelihood method (ML) based on the complete mitochondrial genomic sequences of closely related species to analyze the phylogenetic relationship with related species. The results indicate that the new sweetpotato storage pest is identified as P. gularis (Zeller), with a complete mitochondrial genome length of 15393 bp, an A+T content of 79.67%, and comprising 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes, totaling 37 genes, along with one non-coding control region. Among the 13 protein-coding genes, the start codon of the cox1 gene is CGA, while the start codons of the other genes are typical ATN. The termination codons for the nad2, cox1, cox2, and nad4 genes are incomplete stop codons (T--), whereas the termination codons for the other protein-coding genes are typical TAA or TAG. Of the 22 tRNA, only trnS1-GCT lacks the DHU arm, while the others can form typical cloverleaf structures. Phylogenetic analysis reveals that P. gularis (sweetpotato) is a member of the family Pyralidae (subfamily Galleriinae, superfamily Pyraloidea), closely related to Corcyra cephalonica (Stainton). This study is the first to report that P. gularis (Zeller) damages sweetpotato storage roots, and its distinct morphological characteristics and mitochondrial genomic sequence are clarified, which provide fundamental data for the identification, control, and research of this pest.

Key words: sweetpotato, pest, Paralipsa gularis (Zeller), morphological characteristics, mitochondrial genome

CLC Number:

S435.315

TANG Fajiang, ZHOU Shixu, HUANG Lifei, WANG Zhangying, ZOU Hongda, KE Jie, CHEN Xinliang, ZHANG Shaorun. Identification and Mitochondrial Genome Analysis of a New Stored Pest of Sweetpotato: Paralipsa gularis (Zeller)[J]. Chinese Agricultural Science Bulletin, 2026, 42(9): 197-206.

Figures/Tables 8

References 30

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基因	编码链	位置/bp	长度/bp	反密码子	起始密码子	终止密码子	基因间隔/bp
trnM	J	1~69	69	CAT			0
trnI	J	82~148	67	GAT			12
trnQ	N	150~218	69	TTG			1
nad2	J	282~1293	1012		ATT	T--	63
trnW	J	1294~1361	68	TCA			0
trnC	N	1354~1419	66	GCA			-8
trnY	N	1420~1486	67	GTA			0
cox1	J	<1495~3025	1531		CGA	T--	8
trnL2	J	3026~3092	67	TAA			0
cox2	J	3141~3774	634		ATG	T--	48
trnK	J	3775~3845	71	CTT			0
trnD	J	3845~3910	66	GTC			-1
atp8	J	3911~4075	165		ATC	TAA	0
atp6	J	4069~4749	681		ATG	TAA	-7
cox3	J	4749~5537	789		ATG	TAA	-1
trnG	J	5541~5606	66	TCC			3
nad3	J	5616~5960	345		ATT	TAA	9
trnA	J	5967~6034	68	TGC			6
trnR	J	6034~6095	62	TCG			-1
trnN	J	6101~6166	66	GTT			5
trnS1	J	6218~6283	66	GCT			51
trnE	J	6289~6355	67	TTC			5
trnF	N	6362~6428	67	GAA			6
nad5	N	6434~8152	1719		ATT	TAA	5
trnH	N	8168~8232	65	GTG			15
nad4	N	8233~9571	1339		ATG	T--	0
nad4l	N	9575~9865	291		ATG	TAG	3
trnT	J	9872~9935	64	TGT			6
trnP	N	9936~10000	65	TGG			0
nad6	J	10033~10539	507		ATT	TAA	32
cob	J	10539~11690	1152		ATG	TAA	-1
trnS2	J	11692~11759	68	TGA			1
nad1	N	11779~12714	936		ATG	TAA	19
trnL1	N	12716~12784	69	TAG			1
rrnL	N	12832~14128	1297				47
trnV	N	14161~14227	67	TAC			32
rrnS	N	14228~14985	758				0
CR	J	14986~15393	408				0

基因	编码链	位置/bp	长度/bp	反密码子	起始密码子	终止密码子	基因间隔/bp
trnM	J	1~69	69	CAT			0
trnI	J	82~148	67	GAT			12
trnQ	N	150~218	69	TTG			1
nad2	J	282~1293	1012		ATT	T--	63
trnW	J	1294~1361	68	TCA			0
trnC	N	1354~1419	66	GCA			-8
trnY	N	1420~1486	67	GTA			0
cox1	J	<1495~3025	1531		CGA	T--	8
trnL2	J	3026~3092	67	TAA			0
cox2	J	3141~3774	634		ATG	T--	48
trnK	J	3775~3845	71	CTT			0
trnD	J	3845~3910	66	GTC			-1
atp8	J	3911~4075	165		ATC	TAA	0
atp6	J	4069~4749	681		ATG	TAA	-7
cox3	J	4749~5537	789		ATG	TAA	-1
trnG	J	5541~5606	66	TCC			3
nad3	J	5616~5960	345		ATT	TAA	9
trnA	J	5967~6034	68	TGC			6
trnR	J	6034~6095	62	TCG			-1
trnN	J	6101~6166	66	GTT			5
trnS1	J	6218~6283	66	GCT			51
trnE	J	6289~6355	67	TTC			5
trnF	N	6362~6428	67	GAA			6
nad5	N	6434~8152	1719		ATT	TAA	5
trnH	N	8168~8232	65	GTG			15
nad4	N	8233~9571	1339		ATG	T--	0
nad4l	N	9575~9865	291		ATG	TAG	3
trnT	J	9872~9935	64	TGT			6
trnP	N	9936~10000	65	TGG			0
nad6	J	10033~10539	507		ATT	TAA	32
cob	J	10539~11690	1152		ATG	TAA	-1
trnS2	J	11692~11759	68	TGA			1
nad1	N	11779~12714	936		ATG	TAA	19
trnL1	N	12716~12784	69	TAG			1
rrnL	N	12832~14128	1297				47
trnV	N	14161~14227	67	TAC			32
rrnS	N	14228~14985	758				0
CR	J	14986~15393	408				0

一点缀螟P.gularis(Zeller)	长度/bp	A/%	T/%	G/%	C/%	A+T/%	G+C/%	AT-skew	GC-skew
全线粒体基因组序列Mitogenome	15393	39.34	40.34	7.78	12.55	79.67	20.33	-0.013	-0.235
蛋白编码基因PCGs	11101	32.74	44.92	11.24	11.1	77.66	22.34	-0.157	0.006
tRNA基因tRNAs	1470	41.02	40.41	10.68	7.89	81.43	18.57	0.008	0.15
rRNA基因rRNAs	2055	43.55	40.73	10.51	5.21	84.28	15.72	0.033	0.337
控制区Dloop	408	46.08	49.26	1.23	3.43	95.34	4.66	-0.033	-0.474

一点缀螟P.gularis(Zeller)	长度/bp	A/%	T/%	G/%	C/%	A+T/%	G+C/%	AT-skew	GC-skew
全线粒体基因组序列Mitogenome	15393	39.34	40.34	7.78	12.55	79.67	20.33	-0.013	-0.235
蛋白编码基因PCGs	11101	32.74	44.92	11.24	11.1	77.66	22.34	-0.157	0.006
tRNA基因tRNAs	1470	41.02	40.41	10.68	7.89	81.43	18.57	0.008	0.15
rRNA基因rRNAs	2055	43.55	40.73	10.51	5.21	84.28	15.72	0.033	0.337
控制区Dloop	408	46.08	49.26	1.23	3.43	95.34	4.66	-0.033	-0.474