Abstract: Swertia mussotii Franch is a rare and precious medical herb in China and mainly distributes in Qing-hai Province. Because of the natural resources of Swertia mussotii Franch destroyed seriously, the article researched the rate of germination during the process of artificial introduction and cultivation so as to offer substantial and theoretical foundation for protecting wild Swertia mussotii Franch. The result showed that Swertia mussotii Franch had low rate of fructification in natural state, but dealt with Na2CO3, IAA, low temperature and after ripening, the germination of seeds of Swertia mussotii Franch increased obviously.