Abstract: The 277 varieties and lines, which come from HuangHuai and Yangtse River Area in China, were analyzed using SDS-PAGE for its composition of HMW-GS. The results showed that the 3 subunit types which were 1, 2*, N were screened at Glu-A1 locus. Among the three subunits, the frequency of 1 subunit was the highest, its value was 49%; the second was N subunit, its value was 44.77%, the two subunit’s frequency summed to 94.22%. The allele subunits were most abundance at Glu-B1 locus; there were 8 alleles subunit that were screened. Among the subunits, the frequency of 7+8 and 7+9 was the highest value, 38.99% and 36.10% respectively. The next was 14+15, 12.64%, and other subunit’ frequencies were less. 3 subunit types, which were 2+12, 5+10, 4+12, their frequencies were 44.77%, 33.94%, 19.49%, respectively, were identified at Glu-D1 locus. The 43 subunit compositions were identified in the test varieties, the number of varieties included N, 7+8, 2+12 was the highest, its frequency was 12.64%, the second subunit compositions was N, 7+9, 2+12, which accounted for 9.39%; the frequency of subunit compositions included 1, 7+8, 2+12, which accounted for 7.94%; N, 14+15, 4+12 accounted for 5.42%