Abstract: The experiments were carried out with Guanglingxiangjing and Liangyoupeijiu, the effect of application stage of panicle fertilizer on yield and the quality of rice were studied systematically. The results showed as follows: (1) Different cultivars varied under different application stage of panicle fertilizer. Under 15kg/mu total application amount of nitrogen, Liangyoupeijiu, that was applicated at inverse 1st leaf stage got high yield easily, under 10kg/mu total application amount of nitrogen, it got higher yield at inverse 4th leaf and inverse 2nd leaf stage. Under suitable total application amount of nitrogen, Guanglingxiangjing was coordinated between panicle and ears, enhanced kilo grain weight and seed setting percentage, and enhanced yield at inverse 4th leaf and inverse 2nd leaf stage. (2) Under the same application amount, along with postponing the application stage, the percentage of chalkiness and chalkiness of Liangyoupeijiu were lowered, and the milling quality and the protein content were improved, the amylose content and the gel consistency were decreased, the percentage of chalkiness and the milling quality and the protein content of Guanglingxiangjing, were increased along with postponing the application stage, but the amylase content and gel consistency was decreased. Therefore, to got high yield, the panicle fertilizer of Liangyoupeijiu was applied twice at inverse 3rd leaf and inverse1st leaf stage, and Guanglingxiangjing was at inverse 4th leaf and inverse 2nd leaf stage.