Abstract: In this experiment, 29 materials of three main cultivars in Cucurbita were crossed. The results showed that C. pepo was only suit to be male parent in carrying out interspecific hybridization with C. moschata or C. maxima. There were some compatible differences in different female materials of C. moschata or C. maxima, including setting percentage, seed single fruit, seed development stage and having embryo fruit rate and variations of C. pepo could freely cross to fuse target characters since they didn’t have procreating segregant. Trails showed that the best pollination time was in A.M 6:30-8:30,cultivation techniques of fly net covering in the whole course and slinging vine could improve fruit rate and lengthen embryo development time, which improved compatibility of Cucurbita interspecific hybridization.