Abstract: The runner plants of five strawberry varieties were irradiated by three different doses of 60Co-γ ray, and the irradiation effect of lethality, lesion, growth restraint on runner plants and the production of new runner plants were studied. The results indicated there was the dead runner plant in five strawberry varieties treated by 60Co-γ ray within 30-80Gy. The death rate was between 22% and 88.7%, which raised with increase in the dose of 60Co-γ ray, but there were different death rate from the different variety. There happened abnomal leaves in all treatment. The change of the length and width of leaf, the height and size of plant was different with irradiation dosage and strawberry variety. The occurance rate and the quantity of the new runner decreased significantly. The different varieties had different sensitivity to the irradiation of 60Co-γ ray, the most sensitive variety was “akihime”, the second was “hokowase”, “chunxu” and “shengxing”, “shuofeng” was most insensitive. The half lethal dose of irradiation to “akihime” was respond to between 30Gy and 50Gy, to “hokowase”, “chunxu” and “shengxing” was between 50Gy and 80Gy, to “shuofeng” above 80Gy.