Abstract: CBF1 gene from Arabidopsis thaliana was used to transform Dianthus chinese via agrobacterium mediated method and obtain 4 lines of trandgenic plants. The cold-resistance of transgenic plants were estimated by the relative electrolyte leakage percentage, the content of Malondialdehyde and the prolinecontents. Under different low temperature treatment, the relative electrolyte leakage percentage of transgenic lines was lower than CK, the content of Malondialdehyde (MDA) of transgenic lines was lower than CK, and the prolinecontents of transgenic lines was higher than CK. The result showed that the cold-resistance of Dianthus chinese enhanced after CBF1 gene transformed into Dianthus chinese. Cold-resistance of line 27 was highest in four transgenic lines.