Construction of Plant Leaf-specific Expression Vector with PPDK of [Echinochloa crusgalli var frumentacea(Roxb.)W.F.Wight] and Transfer to Agrobacterium tumefaciens

Abstract

Abstract: 【OBJECTIVE】 The pyruvate-phosphate dikinase expressed effectively by utilizing leaf-specfic promoter of small subunits of rubisco; 【METHOD】The small subunits of rubisco and the gene of Pdk were digested by PstI and SalI from pRGN and pUCm-Pdk,and inserted into expression vector pCAMBI1301.Then it was transformed into EHA105 by direct entering way; 【RESULTS】The plant transformation vector p1301-Prbcs-PPDK was constructed in this experiment. The selective marker gene was Hpt(Hygomycin phosphate transferase). By digestion and PCR identification, The vector p1301-Prbcs-PPDK was transferred to Agrobacterium tumefaciens EHA105 ; 【CONCLUSION】 The pyruvate-phosphate dikinase is the key enzyme of C4 photosynthesis.The experiment constructed a expression vector suit to monocotyledon,which will provide an important experimental base for study on PPDK function..

CLC Number:

Wang Jinming,, Zhao Ming, Ding Zaisong, Zhang Bin, Guo Zhijiang. Construction of Plant Leaf-specific Expression Vector with PPDK of [Echinochloa crusgalli var frumentacea(Roxb.)W.F.Wight] and Transfer to Agrobacterium tumefaciens[J]. Chinese Agricultural Science Bulletin, 2007, 23(5): 63-63.

References

[1] ap Rees T. Hill S A. Metabolic control analysis of plant metabolism. Plant Cell Environ,1994,17:587-599.
[2] Trevanion S J, Furbank R T, Ashton A R. NADP-malate dehydrogenase in the C4 plant Flaveria bidentis. Cosense suppression of activity in mesophyll and bundle-sheath cells and consequences for photosynthesis. Plant Physiol., 1997,113:1153-1165.
[3] Zhang L(张丽). Comparison of photosynthetic characters and their physiological mechanism in C3 and C4 crops. Dissertation of Master degree in China Agricultural University. (中国农业大学硕士学位论文), 2004(in Chinese).
[4] 刘巧泉,于恒秀,张文娟,等.水稻rbcs启动子控制的外源基因在转基因水稻中的特异性表达.植物生理与分子生物学学报(Journal of Plant Physiology and Molecular Biology). 2005, 31(3):247-253.
[5] Matsuoka M. Structure, genetic mapping, and expression of the gene for pyruvate, orthophosphate dikinase from maize. J Biol Chem, 1990, 265(28): 16772-16777.
[6] Sheen J. Molecular mechanisms underlying the differential expression of maize pyruvate, orthophosphate dikinase genes. Plant Cell, 1991,3(3):225-245.
[7] Ishimaru K, I chikawa H, Matsuoka M,Ohsugi R. Analysis of a C4 maize pyruvate, orthophosphate dikinase expressed in C3 transgenic Arabidopsis plants.Plant Sci., 1997,129:57-64.
[8] Fukayama H, Tsuchida H, Agarie S, et al. Significant accumulation of C4-specific pyruvate, orthophosphate dikinase in a C3 plant, rice. Plant Physiology, 2001,127:1136-1146.
[9] Ku M S B, Cho D H, Li X, et al. Introduction of genes encoding C4 photosynthesis enzymes into rice plants physiological consequences Rice Biotechnology. Improving Yield, Stress Tolerance and Grain Quality, 2001, 236:100-116.
[10] Morris S H, Adley C C. Irish public perceptions and attitudes to modern biotechnology: an overview with a focus on GM foods[J]. Trends Biotechnol, 2001,19(2):43.
[11] Kuiper H A, Kleter G A, Noteborn H P, et al. Assessment of the food safety issues related to genetically modified foods[J]. Plant J, 2001,27(6):503.

[1]	Ma Huimin, Sun Peilin, Ma Chunquan. Salt Tolerance Function of Transcription Factor BvM14-GAI [J]. Chinese Agricultural Science Bulletin, 2021, 37(34): 34-42.
[2]	Chen Li, Gu Hui, Jia Zhiwei, Hong Keqian. Dof Transcription Factor in Pineapple Fruits Treated with Hexaldehyde Delaying Internal Browning Resistance: Expression Analysis [J]. Chinese Agricultural Science Bulletin, 2020, 36(15): 115-122.
[3]	Rongtian Li, Shitong Gao, Yi Gao, Changhua Liu. Effect of Bt Early Japonica Rice on Soil Microorganism Quantity and Transfer of Exogenous Genes of Rice [J]. Chinese Agricultural Science Bulletin, 2020, 36(9): 56-64.
[4]	. The Response of Energy Beet BvGST Gene to Cadmium Stress in Escherichia coli [J]. Chinese Agricultural Science Bulletin, 2019, 35(36): 116-121.
[5]	. OsVDAC3 and 3 Interaction Protein Genes OsV3IP1-3 in Rice Cultivars: Expression Pattern Analysis [J]. Chinese Agricultural Science Bulletin, 2019, 35(6): 101-107.
[6]	. Expression Change of Transcription Factors of Rice Under Drought Stress [J]. Chinese Agricultural Science Bulletin, 2019, 35(6): 108-114.
[7]	. 2,3-Butanediol Dehydrogenase Gene (BudC) Overexpress K. oxytoca HD79 Strain: Construction and Identification [J]. Chinese Agricultural Science Bulletin, 2018, 34(27): 89-96.
[8]	程景民 and 胡婧超. Visualized Comparative Analysis of Resistant Transgenic Rice Researches: Between the Global and the Domestic [J]. Chinese Agricultural Science Bulletin, 2018, 34(14): 149-154.
[9]	. Irrigation Amount Affecting Photosynthetic Characteristics and Yield Components of Spring Wheat in Ningxia [J]. Chinese Agricultural Science Bulletin, 2017, 33(34): 9-14.
[10]	. Distribution of Microalgae in Saline Lakes of Heilongjiang and Effects of Physical and Chemical Factors on the Biomass of Dominant Algal Strains [J]. Chinese Agricultural Science Bulletin, 2017, 33(23): 68-75.
[11]	Yu Hang,Gao Dongni,Tian Zhaofeng,Ping Wenxiang and Ge Jingping. Effect of Different Promoters on Expression of HA-VP2 Recombinant Baculovirus Protein [J]. Chinese Agricultural Science Bulletin, 2017, 33(23): 100-105.
[12]	Ge Jingping,Bai Chengle,Gao Dongni,Shen Yan,Yu Hang and Ping Wenxiang. Construction of Chicken IL-2 Recombinant Baculovirus and Its Histopathological Study [J]. Chinese Agricultural Science Bulletin, 2017, 33(21): 142-148.
[13]	. Cell Penetrating Peptides Mediated Transformation of Triticale Microspore [J]. Chinese Agricultural Science Bulletin, 2017, 33(20): 29-34.
[14]	. The Construction of Acetolactate Synthase Gene Integration Expression Vector [J]. Chinese Agricultural Science Bulletin, 2017, 33(8): 25-30.
[15]	程景民 and 李欣彤. Study on Mapping Knowledge Domains of Transgenic Maize: Based on Citespace [J]. Chinese Agricultural Science Bulletin, 2017, 33(5): 128-132.