Abstract: 【OBJECTIVE】The purpose of this research was to provide basis in theory and in technique for planting Yudan 2002 and high-yield and high-protein content maize cultivars. 【METHOD】this study was conducted with five treatments of N0（CK）, N1( 100% shooting fertilizer ), N2 was the worst ( 100% ear fertilizer), N3(40% shooting fertilizer +60% ear fertilizer ), N4 (30% shooting fertilizer +50% ear fertilizer+20% grain fertilizer)in high-yield field in Chao Soil area of north Henan province. 【RESULTS】The results showed that the fertilization effect was different within the treatments arranged, among them the effect of N3 was the highest, then that of N1( 100% shooting fertilizer )and N4 (30% shooting fertilizer +50% ear fertilizer+20% grain fertilizer),the effect of N2 was the lowest ; Nitrogen fertilization more than one time was in favor of the grain protein concentration increase, the more the fertilization time was adopted, the higher the seed protein concentration was got; nitrogen fertilization more than one time was also in favor of the grain protein yield increase, among those treatments, N3 was better than N4; nitrogen management treatments had significantly different nitrogen fertilizer utilization rate, among them N3 got the highest, then the N4 and N1, N2 was the lowest. 【CONCLUSION】N3 was the optimized treatment in that it can accumulate most nitrogen in plant, have significant increase effect in seed yield and seed protein yield, and showed increase trend of seed protein concentration.