Abstract: 【OBJECTIVE】In order to realize the difference in the aroma of flue-cured tobacco between Yunnan and Zimbabwe, guide for the study and production of partial substitution for import flue-cured tobacco in Yunnan, and improve of tobacco quality, the test was in progress. 【METHOD】The content of polyphenols, petrol ether extract, polytomic acid and higher fatty acid, and the volatile aroma of flue-cured tobacco in Yunnan partial substitution for import plant areas of China was determined by colorimetry, classical soxhlet extraction, sulfate methylization, GC-MS combined with simultaneous distillation and solvent extraction, respectively. And these results were analyzed and compared with that of Zimbabwe systematically.【RESULTS】Results showed that the content of petrol ether extract and degradation aromatic products from aromatic amino acid of the leaves at different stalk positions was more than that of Zimbabwe; but the content of polyphenols and higher fatty acid was lower than that of Zimbabwe. The content of cembranoid aroma in the upper leaves of Yunnan was more than that of Zimbabwe; but the content of polytomic acid, degradation aromatic products from carotenoids and Maillard reaction products was lower than that of Zimbabwe. The content of polytomic acid, degradation aromatic products from carotenoids and Maillard reaction products in the central and lower leaves of Yunnan was more than that of Zimbabwe; but the content of cembranoid aroma was lower than that of Zimbabwe. Devious difference of aroma of flue-cured tobacco between Yunnan and Zimbabwe exist in upper leaves, in particular the volatile aroma such as megastigmatrienone, 5,9,13-pentadecatrien-2-one, 6,10,14-trimethyl, 3-buten-1-one, 4-[2,6,6-trimethyl-1(or 2)-cyclohexen-1-yl], 3-furanmethanol, furfural, 2(4H)-benzofuranone, and 5,6,7,7a-tetrahydro-4,4,7a-trimethyl, the nonvolatile aroma such as citric and oleic acid.【CONCLUSION】There was significant in the aroma of flue-cured tobacco between Yunnan and Zimbabwe, and the contene of aroma of upper leaves in Yunnna was less than that of Zimbabwe.