Abstract: 【OBJECTIVE】Pob45,Pob46,Pob501,Pob502,Pool33,Pool34,Pob25 and Suwan1,8 tropical,subtropical maize populations were crossed to analyze their combining ability and heterosis. 【METHOD】Five domestic maize inbreds (Chang7-2,Zheng22,Ye478,Qi319 and Mo17) used as tester by NCⅡ mating design. 【RESULTS】The results showed that the efficiency value of GCA (general combining abality) for Pob25 and Pob502 was higher. Five heterotic patterns have been obtained from the efficiency value of GCA、special combining ability (SCA) and total combining ability (TCA), 【CONCLUSION】Five heteritic patterns have been obtained from this study, they are Tangsipingtou×Pob501, Tangsipingtou×Pob25, Reid×Pob25, Tangsipingtou×Suwan1，Ludahonggu×Pob502.