Abstract: [Abstract] Objective：This research focused on analyzing the differences of psbA-trnH region sequences of Artemisia annua L，Artemisia capillaris Thunb and Artemisia carvifolia Buch, which can provide the species-special character-sites for discriminating them. Method：DNA was extracted from the collected three Artemisia samples and their psbA-trnH regions were amplified by PCR，and then sequenced and analyzed to find the character-sites．Result：Although the psbA-trnH regions among the three species are high similar, there are several character-sites for themselves, which can be used in discriminating them. Conclusion：psbA-trnH region can provide the character-sites used to identify each of the three varieties unequivocally. It is the first time to use DNA technology in drug species’ discrimination of Artemisia, which provided a reliable and simple reference for the authentication of them．