Abstract: Abstract: In order to obtain the simple method of sieving the seeds of transgenic rapeseed tagging NPT-II. Using three methods such as Kanamycin daubing vane, dipping seeds and MS culture medium containing Kanamycin to study. The results indicated that the best Kanamycin choice press was 200 mg/L. The method of Kanamycin daubing vane can distinguish to Km- resistance or not for 4-5 days. Four plants were chosen in this method and its PCR detection was positive. The reliability reached 100%. The method of dipping seeds can distinguish to Km- resistance or not for 2-3 days. Five plants were chosen in this method and its PCR was positive. The reliability reached 100%. The method of Kanamycin MS culture medium can distinguish to Km- resistance or not commonly for 2-3 days. Twenty plants were chosen in this method but only five saved in transplanting. The result of PCR detection is that there plants were positive and two were negative. The reliability reached 60%. So, it could be thought Kanamycin daubing vane and dipping seeds were ideal methods for fast screening transgenic rapeseed and descendant’s heredity analysis.