Abstract:

Abstract： 【OBJECTIVE】The component of plasma is complex and plasma protein present information-rich. The change of structure and quantity of plasma protein is very important for disease diagnosis and treatment; 【METHOD】Two-dimensional electrophoresis is a key protein separation technology in proteomics research. Plasma protein have high dynamic range characterization, and high-abundance proteins can interfere with analysis. So we designed two experiments for study of plasma proteomics in dairy cow, and compared them in order to refer to plasma application as diagnosis marker;【RESULTS】230 protein points were separated which appeared in three repeat-gels by Hot-SDS method and 156 protein points were separated by Aurum Affi-Gel Blue Mini columns method.【CONCLUSION】The method that Hot-SDS and pH4-7 IPG strip were used was better.