Abstract: The genetic male sterile line 01-3AB was selected and bred by using a male sterile mutant of celery discovered in self-cross line 01-3,through means of self-cross,testcross and siblingcross. It has stable sterility inheritanting according to Mendel’s law controlled by a pare of nuclear recessive genes with fine horticultural characters. The gene of male sterility was transferred to six different genetypes. “Jinqi 1” and ‘Jinqi 2”,two hybrid varieties of celery were crossbred by 01-3AB line as female parent.