Abstract: The tissue culture of Fengxiang strawberry was used as experimental materials. The effects of PVA on tissue culture of strawberry were studied. The results indicated that 1g/L PVA can reduce the rate of vitrified plantlets of strawberry significantly during proliferating, and can also improve the contenes of chlorophyll and albumen. During leaf culture, 1g/L PVA may quicken callus emergence and increase the rate of callus formation and improve the quality of callus. 2g/L PVA can improve the root number of single plantlet, the length of roots, the high of plantlet, the contents of chlorophyll and albumen.