Abstract:

Immature male flowers of Musa paradisiaca ABB Linn. were inoculated on embryogenic callus induction media. Yellow granular calli emerged after 4–5 months were shown to be embryogenic calli. And they could be proliferated by long-term subculture. These friable and embryogenic calli had been subcultured for 6 years. After they were transplanted on differentiation medium containing 0.2 mg/L 6-BA, buds could be induced, and 53 regeneration plantlets had been obtained. These plantlets could be propagated further. Histological sections proved that the calli proliferated by long-term subculture maintained the embryogenic character.