Abstract:

The cDNA amplified fragment length polymorphism (cDNA-AFLP) analysis system of Capsicum annuum was established using buds and leaves as materials. The results indicated that Trizol method could be used for extracting ideal RNA from buds and leaves of pepper while double-stranded cDNA could be synthesized from total RNA by SMART method. Double-stranded cDNA was digested completely after 3h digestion by Taq I and Ase I step by step. The reducible result was obtained when the ligation products diluted to 15 times as templates for pre-amplification and 15 times-diluted pre-amplification products for selective amplification. The cDNA-AFLP analysis system was established successfully, which provided platform for further molecular study and gene cloning of Capsicum annuum.