Abstract:

Studied petals of Fragaria orientalis Lozinsk. var. concolor (Kitag.) Liou et C. Y. Li which were used as explants and the main factors with different levels through uniform design experiments for callus induction and variation plants from callus. The results showed that the eleven proper culture medium for callus induction, with the induction rates of callus were more than 65.0%, LS+TDZ 2.06 mg?L-1 +2,4-D 0.60 mg?L-1 was the most suitable for callus induction from petals by combinatorial optimization, with which the frequency of callus induction was 98.0%; ten medium were suitable for shoots redifferentiation from callus induced by the medium of LS + TDZ 2.20 mg?L-1 +2,4-D0.40 mg?L-1, 1/2 LS+TDZ 2.30 mg?L-1 + KT 1.38 mg?L-1 was the most suitable for callus redifferentiation by combinatorial optimization, with which the frequency of differentiation was 96.3%, the frequency of variation was 2.77% through field experiments. Screen six variation lines through 5-year field experiments form shoots which were produced from ten medium with various hormones with concentration ratio and one medium by optimization. It shows that the petals of Fragaria orientalis Lozinsk. var. concolor (Kitag.) Liou et C. Y. Li was possible to induct variation plants under suitable hormones with concentration ratio conditions, and tend to variation with steady properties.