Abstract:

New tomato varioties T431, 1911 and 0701 were used as the materials, chitinase gene and β-1,3-glucanase gene were transformed into tomatoes by Agrobacterium-mediated transformation. A total of 100 regeneration plants were obtained. In these regenerated plants, 27 and 13 plants appeared respectively to be positive in PCR test with chitinase gene and β-1,3-glucanase gene primer. The transferring frequency of foreign gene was 27% and 13% separately. If the two genes were done simultaneously, 10 plants appeared to be positive in PCR test with a transferring frequency of 10%. PCR-Southern analysis of these plants showed that they produced positive hybridization ,indicating that the chitinase gene and β-1,3-glucanase gene have been conveyed and integrated into genome of these plants.9 plants chosen from double-gene transgenic plants were taken RT-PCR analysis,5 and 4 plants appeared respectively to be positive in 9 plants, which proved that the chitinase gene and β-1,3-glucanase gene acquired stable expression in transgenic plants.