Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (8): 234-236.

Special Issue: 农业地理

• 23 • Previous Articles     Next Articles

Microsatellite markers for genetic polymorphism in five geographic population of the Bactrocera dorsalis (Hendel)

Yao Tingshan, Hu Junhua, Li Hongjun, Ran Chun, Liu Haoqiang, Lei Huide, Xiao Tia   

  • Received:2009-10-14 Revised:2009-11-04 Online:2010-04-20 Published:2010-04-20

Abstract:

Abstract: The fruits flies Bactrocera (Bactrocera) dorsalis (Hendel) is one of the major economically important and quarantine insects in Asia. Genomic DNA of the sampled populations came from Fujian, Hainan, Guangdong, Yunnan and Sichuan,was extracted from thoracic tissue of individual adult ?ies by CTAB method and amplified with the polymerase chain reaction. By using microsatellite markers (SSR), the fingerprint of five geographic population was investigated. With good reproducibility and high polymorphic, seven microsatellite primers were utilized in this study to assess the genetic polymorphism of orient fruit fly primarily. The results indicated that genetic differentiation exists among the five populations.