Abstract:

The aim of our experiment was to investigate molecular immunological characters of VP1 in foot-and-mouth disease virus from OS/99 viral strain cultured in BHK-21 cells . In this study,the sequence of VP1 ,which was isolated through RT-PCR , was analyzed in DNAstar software to abtain its spatial arrange and antigenicity . The result of this research indicated that the VP1 from OS/99 might contain many antigenic sites in terms of some parameters on the antigenicity , which located in 22-29，43-46，82-85，92-93，100-111，134-139，154-156，180-184，196-203 amino acid regions . For that , the virus strain of os/99 showed the powerful ability in aspect of more broader coverage in antigenicity Together ,it was suggested that the spatial conformation of VP1 in OS/99 virus strain ,which were subcultured for 13 generations , have not been obviously changed .