Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (10): 260-265.

Special Issue: 生物技术 园艺

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Cloning and Sequencing of CHLH Gene in Fragaria pentaphylla Lozinsk

  

  • Received:2011-01-05 Revised:2011-02-21 Online:2011-05-15 Published:2011-05-15

Abstract:

The aim was to study the functions of abscisic acid binding protein in straw berry resistance. Therefore, the research of abscisic acid binding protein was the crux of the problem in the mechanism of ABA. In this study, the total DNA was extracted from Fragaria pentaphylla Lozinsk leaves, and the cDNA sequence was amplified through PCR. The results showed gene had 4662 bp in length, encoding 1393 amino acid. The CHLH has high sequence homology with other plants,it shared 77%-90%identity in nucleotide sequence and 57%-77% identity in amino acid sequence.