Abstract:

Abstract: Objective：to elucidate the genotype distributions of ESBLs produced by E.coli isolated from the fowl in Liaoning Province. Method: thirty-two ESBL-producing Escherichia coli were collected from four different chicken farms in Dalian, and were analyzed by PCR amplifying using the primers of TEM, OXA, SHV and CTX-M, respectively. Result: thirty-two strains with TEM-type, twelve CTX-M-types and two OXA-types were detected except SHV-type was not found; moreover, eleven strains with multiple TEM+CTX-M and two strains with TEM+OXA genotyes were detected. Conclusion: the predominant genotype of ESBLs is TEM (100% detection rate) in chicken farms of Liaoning Province, and then are CTX-M, SHV was not found; the multiple TEM+CTX-M positive rate reached up to 34.4％.