Abstract:

Abstract:The CDV nucleocapsid protein gene was cloned into pMD-18T vector, restriction analysis was positive for plasmid DNA sequencing. The pMD-18T-N was digested by EcoRⅠand KpnⅠand cloned into the eukaryotic expression vector pcDNA, the positive clones pcDNA-N was screening by electrophoresis sequencing, then transfected COS-7 cells and verified with the IFA. Intraperitoneal injection of 8-week-old BABL /c mice after the plasmid pcDNA-N emulsified with Freund's adjuvant. At same time the original vector as negative control pcDNA. A total of three inoculations were performed at once every two weeks.Two weeks after the last injection, blood samples were collected to separate serum. Results of ELISA show that titer stimulated by pcDNA-N is 101.62±0.164. While the control group’s titer is 100.52±0.56 , can not been detected CDV antibody. These experiments provided primary data for in vivo research of dogs.